The effects of SDS at subsolubilizing concentrations on the planar lipid bilayer permeability: Two kinds of current fluctuations

被引:17
作者
Anosov, A. A. [1 ]
Smirnova, E. Yu. [1 ]
Korepanova, E. A. [1 ]
Shogenov, I. M. [1 ]
机构
[1] Sechenov Univ, Sechenov First Moscow State Med Univ, 8-2 Trubetskaya St, Moscow 119991, Russia
关键词
Bilayer permeability; Hydrophilic pore; Toroidal pore; BLM of asolectin; SOS-induced pores; Pore energy; Edge energy; SODIUM DODECYL-SULFATE; MOLECULAR-DYNAMICS; MEMBRANES; EVOLUTION; SURFACTANTS; MONOLAYER; VESICLES;
D O I
10.1016/j.chemphyslip.2018.11.005
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Detergent effects on lipid bilayers of artificial and biological membranes at subsolubilizing concentrations are known to include the membrane permeabilization which manifests itself through both a flip-flop of detergent molecules from the outer monolayer to the inner monolayer and the membrane leakage of entrapped solutes. We have explored the current fluctuations occurring in planar BLM of asolectin in the presence of ionic detergent SDS at subsolubilizing concentration. Two groups of current fluctuations which the average duration differs by two orders of magnitude can be distinguished. We assume that these differences in the duration of current fluctuations are associated with a different number of SDS molecules in the walls of the putative toroidal hydrophilic pores. We associated short pulses with the formation of short-lived lipid hydrophilic pores. Impulses of greater duration (steps) were associated with the formation of hydrophilic pores, the walls of which contain detergent. Taking into account the characteristics of these pores, we estimated the pore energy, as well as the edge energy of these two kinds of pores. We believe that the flip-flop of SDS molecules in liposomes is provided by long-lived pores, and the contents of the liposome leakage occurs through all pores.
引用
收藏
页码:10 / 15
页数:6
相关论文
共 25 条
[1]   Detergent Effects on Membranes at Subsolubilizing Concentrations: Transmembrane Lipid Motion, Bilayer Permeabilization, and Vesicle Lysis/Reassembly Are Independent Phenomena [J].
Ahyayauch, Hasna ;
Bennouna, Mohammed ;
Alonso, Alicia ;
Goni, Felix M. .
LANGMUIR, 2010, 26 (10) :7307-7313
[2]   INCREASE IN SIZE OF SONICATED PHOSPHOLIPID-VESICLES IN THE PRESENCE OF DETERGENTS [J].
ALONSO, A ;
SAEZ, R ;
VILLENA, A ;
GONI, FM .
JOURNAL OF MEMBRANE BIOLOGY, 1982, 67 (01) :55-62
[3]   Erlang flow of hydrophilic pore formation and closure events in a lipid bilayer during phase transition resulting from diffusion in the radius space [J].
Anosov, A. A. ;
Sharakshane, A. A. ;
Smirnova, E. Yu. ;
Nemchenko, O. Yu. .
EUROPEAN BIOPHYSICS JOURNAL WITH BIOPHYSICS LETTERS, 2018, 47 (03) :297-307
[4]   PEG blocking of single pores arising on phase transitions in unmodified lipid bilayers [J].
Antonov V.F. ;
Smirnova E.Yu. ;
Anosov A.A. ;
Norik V.P. ;
Nemchenko O.Yu. .
Biophysics, 2008, 53 (5) :390-395
[5]   Soft perforation of planar bilayer lipid membranes of dipalmitoylphosphatidylcholine at the temperature of the phase transition from the liquid crystalline to the gel state [J].
Antonov, VF ;
Anosov, AA ;
Norik, VP ;
Smirnova, EY .
EUROPEAN BIOPHYSICS JOURNAL WITH BIOPHYSICS LETTERS, 2005, 34 (02) :155-162
[6]   Molecular dynamics study of the effect of surfactant on a biomembrane [J].
Bandyopadhyay, S ;
Shelley, JC ;
Klein, ML .
JOURNAL OF PHYSICAL CHEMISTRY B, 2001, 105 (25) :5979-5986
[7]   INTERACTION OF DETERGENTS WITH BILAYER LIPID-MEMBRANES [J].
BANGHAM, JA ;
LEA, EJA .
BIOCHIMICA ET BIOPHYSICA ACTA, 1978, 511 (03) :388-396
[8]   THE SHAPE OF LIPID MOLECULES AND MONOLAYER MEMBRANE-FUSION [J].
CHERNOMORDIK, LV ;
KOZLOV, MM ;
MELIKYAN, GB ;
ABIDOR, IG ;
MARKIN, VS ;
CHIZMADZHEV, YA .
BIOCHIMICA ET BIOPHYSICA ACTA, 1985, 812 (03) :643-655
[9]   THEORY OF ELECTROPORATION OF PLANAR BILAYER-MEMBRANES - PREDICTIONS OF THE AQUEOUS AREA, CHANGE IN CAPACITANCE, AND PORE-PORE SEPARATION [J].
FREEMAN, SA ;
WANG, MA ;
WEAVER, JC .
BIOPHYSICAL JOURNAL, 1994, 67 (01) :42-56
[10]   REVERSIBLE ELECTRICAL BREAKDOWN OF LIPID BILAYERS - FORMATION AND EVOLUTION OF PORES [J].
GLASER, RW ;
LEIKIN, SL ;
CHERNOMORDIK, LV ;
PASTUSHENKO, VF ;
SOKIRKO, AI .
BIOCHIMICA ET BIOPHYSICA ACTA, 1988, 940 (02) :275-287