CTRP9 induces iNOS expression through JAK2/STAT3 pathway in Raw 264.7 and peritoneal macrophages

被引：12
作者：
Chen, Ji-Ying [1 ,2 ]
Lei, Sheng-Yun [1 ,2 ]
Li, Ting-Ting [1 ]
Li, Jun [1 ,2 ]
Zuo, An-Ju [1 ,2 ]
Xu, Dan [1 ,2 ]
Song, Cheng-Xiang [2 ,3 ]
Guo, Yuan [1 ]
机构：
[1] Shandong Univ, Dept Gen Practice, Qilu Hosp, 107 Wenhuaxi Rd, Jinan 250012, Peoples R China
[2] Shandong Univ, State & Shandong Prov Joint Key Lab Translat Card, Key Lab Cardiovasc Remodeling & Funct Res,Chinese, Qilu Hosp,Chinese Minist Educ,Chinese Natl Hlth C, 107 Wenhuaxi Rd, Jinan 250012, Peoples R China
[3] Shandong Univ, Dept Cardiol, Qilu Hosp, Jinan, Peoples R China
来源：
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2020年 / 523卷 / 01期
基金：
中国国家自然科学基金;
关键词：
CTRP9; Macrophage; iNOS; JAK2; STAT3; INFLAMMATORY RESPONSE; MYOCARDIAL INJURY; NITRIC-OXIDE; T-CELLS; PROTEIN-9; ACTIVATION; CYTOKINE; LPS; HYPERTROPHY; INHIBITION;
D O I：
10.1016/j.bbrc.2019.12.008
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The Clq tumor necrosis factor (TNF)-related proteins 9 (CTRP9), an adipocyte-derived cytokine, affects a number of physiological processes, including immune function and inflammation. We investigated whether CTRP9 affects the expression of inflammation-related genes in Raw 264.7 and peritoneal macrophages. The CTRP9-induced expression of iNOS increased in a time- and dose-dependent manner. LPS and CTRP9 promote the expression of iNOS jointly in Raw 264.7 and peritoneal macrophages. CTRP9 induced the phosphorylation of JAK2 and STAT3 in Raw 264.7 and peritoneal macrophages. VX509 (JAK2 inhibitor) reduced the CTRP9-induced iNOS protein production. In addition, the CTRP9-induced phosphorylation of JAK2 and STAT3 was dramatically reduced by VX509. Collectively, these results suggest that JAK2/STAT3 signaling is involved in the CTRP9-induced expression of iNOS. (C) 2019 Elsevier Inc. All rights reserved.
引用
收藏
页码：98 / 104
页数：7
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