The LC3 recruitment mechanism is separate from Atg9L1-dependent membrane formation in the autophagic response against Salmonella

被引：141

作者：

Kageyama, Shun ^{[1
]}

Omori, Hiroko

Saitoh, Tatsuya ^{[2
,3
]}

Sone, Takefumi ^{[4
]}

Guan, Jun-Lin ^{[5
]}

Akira, Shizuo ^{[2
,3
]}

Imamoto, Fumio ^{[4
]}

Noda, Takeshi ^{[1
,6
]}

Yoshimori, Tamotsu ^{[1
,6
]}

机构：

[1] Osaka Univ, Grad Sch Frontier Biosci, Lab Intracellular Membrane Dynam, Suita, Osaka 5650871, Japan

[2] Osaka Univ, Microbial Dis Res Inst, WPI Immunol Frontier Res Ctr IFReC, Host Def Lab, Suita, Osaka 5650871, Japan

[3] Osaka Univ, Microbial Dis Res Inst, Dept Host Def, Suita, Osaka 5650871, Japan

[4] Osaka Univ, Microbial Dis Res Inst, Dept Mol Biol, Suita, Osaka 5650871, Japan

[5] Univ Michigan, Sch Med, Dept Internal Med MMG, Ann Arbor, MI 48109 USA

[6] Osaka Univ, Grad Sch Med, Dept Genet, Suita, Osaka 5650871, Japan

来源：

MOLECULAR BIOLOGY OF THE CELL | 2011年 / 22卷 / 13期

关键词：

PHOSPHATIDYLINOSITOL; 3-KINASE; MAMMALIAN AUTOPHAGY; EPITHELIAL-CELLS; HOST-CELLS; BECLIN; PROTEIN; COMPLEX; SYSTEM; LIPIDATION; STARVATION;

D O I：

10.1091/mbc.E10-11-0893

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Salmonella develops into resident bacteria in epithelial cells, and the autophagic machinery (Atg) is thought to play an important role in this process. In this paper, we show that an autophagosome-like double-membrane structure surrounds the Salmonella still residing within the Salmonella-containing vacuole (SCV). This double membrane is defective in Atg9L1- and FAK family-interacting protein of 200 kDa (FIP200)-deficient cells. Atg9L1 and FIP200 are important for autophagy-specific recruitment of the phosphatidylinositol 3-kinase (PI3K) complex. However, in the absence of Atg9L1, FIP200, and the PI3K complex, LC3 and its E3-like enzyme, the Atg16L complex, are still recruited to Salmonella. We propose that the LC3 system is recruited through a mechanism that is independent of isolation membrane generation.

引用

页码：2290 / 2300

页数：11

共 52 条

[1] Salmonella-Containing Vacuoles: Directing Traffic and Nesting to Grow [J].