Rapid measurement of total antioxidant capacity in plants

被引：190
作者：
Gillespie, Kelly M. [1 ]
Chae, June M. [2 ]
Ainsworth, Elizabeth A. [1 ,2 ]
机构：
[1] Univ Illinois, Inst Genom Biol, Program Physiol & Mol Plant Biol, Urbana, IL 61801 USA
[2] USDA ARS, Photosynthes Res Unit, Urbana, IL 61801 USA
来源：
NATURE PROTOCOLS | 2007年 / 2卷 / 04期
关键词：
D O I：
10.1038/nprot.2007.100
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
There is growing interest in measuring the antioxidant status of plant tissues. This protocol describes the oxygen radical absorbance capacity (ORAC) assay, which measures antioxidant inhibition of peroxyl radical-induced oxidations and is a measure of total antioxidant capacity. The assay is performed in a microplate and is assessed with a 96-well multi-detection plate reader. Total antioxidant capacity of 64 experimental samples can easily be analyzed in 1 d. This assay is presented along with rapid assays for total phenolic content and total ascorbate content. Overall, these assays provide a general diagnostic tool of the antioxidant capacity in leaf tissue extracts.
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收藏
页码：867 / 870
页数：4
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