A novel ISFET-based NAD+-dependent enzyme sensor for lactate

被引：43

作者：

Kharitonov, AB ^{[1
]}

Zayats, M ^{[1
]}

Alfonta, L ^{[1
]}

Katz, E ^{[1
]}

Willner, I ^{[1
]}

机构：

[1] Hebrew Univ Jerusalem, Inst Chem, IL-91904 Jerusalem, Israel

来源：

SENSORS AND ACTUATORS B-CHEMICAL | 2001年 / 76卷 / 1-3期

关键词：

NAD(+)-dependent enzyme; impedance spectroscopy; chronopotentiometry; biosensor; field-effect transistor; ISFET; ENFET; lactate; pyrroloquinoline quinone; PQQ;

D O I：

10.1016/S0925-4005(01)00640-2

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

An integrated NAD(+)-dependent enzyme field-effect transistor (ENFET) device for the biosensing of lactate is described. The aminosiloxane-functionalized gate interface is modified with pyrroloquinoline quinone (PQQ) that acts as a catalyst for the oxidation of NADH. A synthetic amino-derivative of NAD(+) is covalently linked to the PQQ monolayer. Faradaic impedance spectroscopy and chronopotentiometry are used as electrochemical methods for probing in situ bioaffinity interaction between NAD+-dependent enzyme, lactate dehydrogenase (LDH) (E.C. 1.1.1.27), and the NAD(+)-functionalized Au-electrode surface. The association constant of 1.5 x 10(5) M-1 was found by both techniques. The affinity complex formed between the NAD(+)/PQQ-assembly and the LDH is cross-linked and yields an integrated biosensor ENFET-device for the analysis of lactate. The device reveals the lower detection limit of 1 x 10(-4) M for lactate and the sensitivity of 24 +/- 2 mV dec(-1). The response time of the device is as low as 15 s. (C) 2001 Elsevier Science B.V. All rights reserved.

引用

页码：203 / 210

页数：8

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