Genome mining for the discovery of new nitrilases in filamentous fungi

被引：30

作者：

Kaplan, Ondrej ^{[1
]}

Bezouska, Karel ^{[1
,3
]}

Malandra, Anna ^{[1
,4
]}

Vesela, Alicja B. ^{[1
]}

Petrickova, Alena ^{[1
]}

Felsberg, Juergen ^{[2
]}

Rinagelova, Anna ^{[1
]}

Kren, Vladimir ^{[1
]}

Martinkova, Ludmila ^{[1
]}

机构：

[1] Acad Sci Czech Republic, Inst Microbiol, Ctr Biocatalysis & Biotransformat, CR-14220 Prague, Czech Republic

[2] Acad Sci Czech Republic, Inst Microbiol, Ctr DNA Sequencing, CR-14220 Prague, Czech Republic

[3] Charles Univ Prague, Dept Biochem, Fac Sci, Prague 12840, Czech Republic

[4] Univ Aquila, Dept Chem Chem Engn & Mat, I-67100 Laquila, Italy

来源：

BIOTECHNOLOGY LETTERS | 2011年 / 33卷 / 02期

关键词：

Aromatic nitrilase; Arylacetonitrilase; Aspergillus niger; Gibberella moniliformis; Neurospora crassa; HYDROLYZING ENZYMES; PURIFICATION; CLONING; VERSATILE; GENE;

D O I：

10.1007/s10529-010-0421-7

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Our aim is to describe new fungal nitrilases whose sequences were published but whose catalytic properties were unknown. We adapted for expression in E. coli three of the genes and confirmed that the enzymes acted on organic nitriles. The genome mining approach was used to search for nitrilases in filamentous fungi. Synthetic genes encoding nitrilases in Aspergillus niger, Gibberella moniliformis and Neurospora crassa were expressed in Escherichia coli. This is the first heterologous expression of fungal enzymes of this type. The recombinant enzyme derived from G. moniliformis was an aromatic nitrilase with an activity of 390 U l(-1) culture with benzonitrile as substrate. This was much less than the activities of the recombinant enzymes derived from A. niger and N. crassa that had activities of 2500 and 2700 U l(-1) culture, respectively, with phenylacetonitrile as substrate.

引用

页码：309 / 312

页数：4