Quantitative Photo Activated Localization Microscopy: Unraveling the Effects of Photoblinking

被引：202

作者：

Annibale, Paolo ^{[1
]}

Vanni, Stefano ^{[2
]}

Scarselli, Marco ^{[1
]}

Rothlisberger, Ursula ^{[2
]}

Radenovic, Aleksandra ^{[1
]}

机构：

[1] Ecole Polytech Fed Lausanne, Inst Bioengn, Lab Nanoscale Biol, Lausanne, Switzerland

[2] Ecole Polytech Fed Lausanne, Lab Computat Chem & Biochem, Inst Chem Sci & Engn, Lausanne, Switzerland

来源：

PLOS ONE | 2011年 / 6卷 / 07期

基金：

瑞士国家科学基金会;

关键词：

SINGLE MOLECULES; DYE MOLECULES; SUPERRESOLUTION; PROTEINS; DYNAMICS; COLOCALIZATION; KINETICS; BLINKING; LIMIT; GFP;

D O I：

10.1371/journal.pone.0022678

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

In this work we discuss how to use photophysical information for improved quantitative measurements using Photo Activated Localization Microscopy (PALM) imaging. We introduce a method that reliably estimates the number of photoblinking molecules present in a biological sample and gives a robust way to quantify proteins at the single-cell level from PALM images. We apply this method to determine the amount of beta 2 adrenergic receptor, a prototypical G Protein Coupled Receptor, expressed on the plasma membrane of HeLa cells.

引用

页数：8

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