Quantitative Photo Activated Localization Microscopy: Unraveling the Effects of Photoblinking

被引：200
作者：
Annibale, Paolo [1 ]
Vanni, Stefano [2 ]
Scarselli, Marco [1 ]
Rothlisberger, Ursula [2 ]
Radenovic, Aleksandra [1 ]
机构：
[1] Ecole Polytech Fed Lausanne, Inst Bioengn, Lab Nanoscale Biol, Lausanne, Switzerland
[2] Ecole Polytech Fed Lausanne, Lab Computat Chem & Biochem, Inst Chem Sci & Engn, Lausanne, Switzerland
来源：
PLOS ONE | 2011年 / 6卷 / 07期
基金：
瑞士国家科学基金会;
关键词：
SINGLE MOLECULES; DYE MOLECULES; SUPERRESOLUTION; PROTEINS; DYNAMICS; COLOCALIZATION; KINETICS; BLINKING; LIMIT; GFP;
D O I：
10.1371/journal.pone.0022678
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
In this work we discuss how to use photophysical information for improved quantitative measurements using Photo Activated Localization Microscopy (PALM) imaging. We introduce a method that reliably estimates the number of photoblinking molecules present in a biological sample and gives a robust way to quantify proteins at the single-cell level from PALM images. We apply this method to determine the amount of beta 2 adrenergic receptor, a prototypical G Protein Coupled Receptor, expressed on the plasma membrane of HeLa cells.
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页数：8
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