Chemokine profile of synovial fluid from normal, osteoarthritis and rheumatoid arthritis patients: CCL25, CXCL10 and XCL1 recruit human subchondral mesenchymal progenitor cells

被引：87

作者：

Endres, M. ^{[1
,2
]}

Andreas, K. ^{[2
,3
]}

Kalwitz, G. ^{[1
,2
]}

Freymann, U. ^{[1
]}

Neumann, K. ^{[1
]}

Ringe, J. ^{[2
,3
]}

Sittinger, M. ^{[2
,3
]}

Haeupl, T.

Kaps, C. ^{[1
]}

机构：

[1] TransTissue Technol GmbH, D-10117 Berlin, Germany

[2] Charite, Dept Rheumatol & Clin Immunol, Tissue Engn Lab, D-10117 Berlin, Germany

[3] Charite, Berlin Brandenburg Ctr Regenerat Therapies BCRT, D-10117 Berlin, Germany

来源：

OSTEOARTHRITIS AND CARTILAGE | 2010年 / 18卷 / 11期

关键词：

Cartilage regeneration; Synovial fluid; Microfracture; Stem cells; Osteoarthritis; Chemokine; ARTICULAR-CARTILAGE DEFECTS; GENE-EXPRESSION PROFILE; STEM-CELLS; CHONDROGENIC DIFFERENTIATION; BONE; RECEPTORS; MIGRATION; REPAIR; MATRIX; CLASSIFICATION;

D O I：

10.1016/j.joca.2010.08.003

中图分类号：

R826.8 [整形外科学]; R782.2 [口腔颌面部整形外科学]; R726.2 [小儿整形外科学]; R62 [整形外科学（修复外科学）];

学科分类号：

摘要：

Objective: The microfracture technique activates mesenchymal progenitors that enter the cartilage defect and form cartilage repair tissue. Synovial fluid (SF) has been shown to stimulate the migration of subchondral progenitors. The aim of our study was to determine the chemokine profile of SF from normal, rheumatoid arthritis (RA) and osteoarthritis (OA) donors and evaluate the chemotactic effect of selected chemokines on human subchondral progenitor cells. Method: Chemokine levels of SF were analyzed using human chemokine antibody membrane arrays. The chemotactic potential of selected chemokines on human mesenchymal progenitors derived from subchondral cortico-spongious bone was tested using 96-well chemotaxis assays. Chemokine receptor expression of subchondral progenitors was assessed by real-time gene expression analysis and immunohistochemistry. Results: Chemokine antibody array analysis showed that SF contains a broad range of chemokines. Ten chemokines that showed significantly reduced levels in RA or OA compared to normal SF or robustly high levels in all SF tested were used for further chemotactic analysis. Chemotaxis assays showed that the chemokines MDC/CCL22, CTACK/CCL27, ENA78/CXCL5 and SDF1 alpha/CXCL12 significantly inhibited migration of progenitors, while TECK/CCL25, IP10/CXCL10 and Lymphotactin/XCL1 effectively stimulated cell migration. MCP1/CCL2, Eotaxin2/CCL24 and NAP2/CXCL7 showed no chemotactic effect on subchondral progenitors. Gene expression and immuno-histochemical analysis of corresponding chemokine receptors document presence of low levels of chemokine receptors in subchondral progenitors, with the CXCL10 receptor CXCR3 showing the highest expression level. Conclusion: These results suggest that SF contains chemokines that may contribute to the recruitment of human mesenchymal progenitors from the subchondral bone in microfracture. (C) 2010 Osteoarthritis Research Society International. Published by Elsevier Ltd. All rights reserved.

引用

页码：1458 / 1466

页数：9

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