Effect of aspartate and glutamate on the fate of enhanced biological phosphorus removal process and microbial community structure

被引：29
作者：
Zengin, Gulsum Emel [1 ]
Artan, Nazik [1 ]
Orhon, Derin [1 ,2 ]
Satoh, Hiroyasu [3 ]
Mino, Takashi [3 ]
机构：
[1] Istanbul Tech Univ, Fac Civil Engn, Dept Environm Engn, TR-34469 Istanbul, Turkey
[2] Turkish Acad Sci, TR-06550 Ankara, Turkey
[3] Univ Tokyo, Grad Sch Frontier Sci, Inst Environm Studies, Chiba 2778563, Japan
来源：
BIORESOURCE TECHNOLOGY | 2011年 / 102卷 / 02期
关键词：
Enhanced biological phosphorus removal; Aspartate utilization; Glutamate utilization; Actinobacterial PAOs; Rhodocyclus-related PAOs; POLYPHOSPHATE-ACCUMULATING ORGANISMS; TARGETED OLIGONUCLEOTIDE PROBES; SEQUENCING BATCH REACTOR; IN-SITU IDENTIFICATION; ACTIVATED-SLUDGE; ANAEROBIC METABOLISM; SUBSTRATE UPTAKE; CARBON-SOURCES; ECOPHYSIOLOGY; COMBINATION;
D O I：
10.1016/j.biortech.2010.09.023
中图分类号：
S2 [农业工程];
学科分类号：
0828 ;
摘要：
This study investigated the fate of enhanced biological phosphorus removal (EBPR) and changes in microbial speciation in a sequencing batch reactor (SBR) fed with aspartate and glutamate. It involved SBR operation for 288 days, batch tests for observation of metabolic functions together with microscopic and phylogenetic analyses. Polyphosphate accumulating organisms (PAOs) were observed in abundance with complete removal of phosphorus. Fluorescence in situ hybridization (FISH) combined with 4',6-dia-midino-2-phenylindole (DAPI) staining confirmed the accumulation of polyphosphate by Rhodocyclus-related and Actinobacterial PAOs. Aspartate seemed to favor the competitive growth of Rhodocyclus-related PAOs since EBPR population used the common biochemical pathways followed by Rhodocyclus-related PAOs in the aspartate fed batch tests. In the glutamate fed batch reactors, however, Actinobacterial PAOs appeared to be competitively selected which explains the lower levels of PHA generation. Even though operational conditions did not change, effective EBPR could not be maintained during the latter part of the study. (C) 2010 Elsevier Ltd. All rights reserved.
引用
收藏
页码：894 / 903
页数：10
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