Recombinase Polymerase Amplification-Based Assay to Diagnose Giardia in Stool Samples

被引：58

作者：

Crannell, Zachary Austin ^{[1
]}

Cabada, Miguel Mauricio ^{[2
]}

Castellanos-Gonzalez, Alejandro ^{[3
]}

Irani, Ayesha ^{[3
]}

White, Arthur Clinton ^{[3
]}

Richards-Kortum, Rebecca ^{[1
]}

机构：

[1] Rice Univ, Bioengn, Houston, TX 77030 USA

[2] Univ Peruana Cayetano Heredia, Dept Internal Med, Cuzco, Peru

[3] Univ Texas Med Branch, Dept Internal Med, Galveston, TX 77555 USA

来源：

AMERICAN JOURNAL OF TROPICAL MEDICINE AND HYGIENE | 2015年 / 92卷 / 03期

基金：

美国国家卫生研究院;

关键词：

HUMAN FECAL SPECIMENS; DNA; CRYPTOSPORIDIUM; PCR; CHILDREN; DISEASE; LAMBLIA; BURDEN; PARVUM;

D O I：

10.4269/ajtmh.14-0593

中图分类号：

R1 [预防医学、卫生学];

学科分类号：

1004 ; 120402 ;

摘要：

Giardia duodenalis is one of the most commonly identified parasites in stool samples. Although relatively easy to treat, giardiasis can be difficult to detect as it presents similar to other diarrheal diseases. Here, we present a recombinase polymerase amplification-based Giardia (RPAG) assay to detect the presence of Giardia in stool samples. The RPAG assay was characterized on the bench top using stool samples spiked with Giardia cysts where it showed a limit-of-detection nearly as low as the gold standard polymerase chain reaction assay. The RPAG assay was then tested in the highlands of Peru on 104 stool samples collected from the surrounding communities where it showed 73% sensitivity and 95% specificity against a polymerase chain reaction and microscopy composite gold standard. Further improvements in clinical sensitivity will be needed for the RPAG assay to have clinical relevance.

引用

页码：583 / 587

页数：5

共 24 条

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