Plasmodium falciparum susceptibility to standard and potential anti-malarial drugs in Dakar, Senegal, during the 2013-2014 malaria season

被引：23

作者：

Fall, Becaye ^{[1
]}

Camara, Cheikhou ^{[2
]}

Fall, Mansour ^{[3
]}

Nakoulima, Aminata ^{[4
]}

Dionne, Pierre ^{[5
]}

Diatta, Bakary ^{[3
]}

Dieme, Yaya ^{[1
]}

Wade, Boubacar ^{[6
]}

Pradines, Bruno ^{[1
,7
,8
,9
]}

机构：

[1] Hop Principal Dakar, Federat Labs, Lab Etud Chimiosensibilite Paludisme, Dakar, Senegal

[2] Hop Principal Dakar, Serv Urgences, Dakar, Senegal

[3] Hop Principal Dakar, Serv Reanimat Med, Dakar, Senegal

[4] Hop Principal Dakar, Serv Pediat, Dakar, Senegal

[5] Maternite Hop Principal Dakar, Dakar, Senegal

[6] Hop Principal Dakar, Chefferie, Dakar, Senegal

[7] Inst Rech Biomed Armees, Unite Parasitol & Entomol, Dept Malad Infect, Bretigny Sur Orge, France

[8] Aix Marseille Univ, Unite Rech Malad Infect & Trop Emergentes, CNRS 7278, IRD 198,Inserm 1095,UM 63, Marseille, France

[9] Ctr Natl Reference Paludisme, Marseille, France

来源：

MALARIA JOURNAL | 2015年 / 14卷

关键词：

Malaria; Plasmodium falciparum; Anti-malarial; In vitro; Resistance; Senegal; Proveblue; Methylene blue;

D O I：

10.1186/s12936-015-0589-3

中图分类号：

R51 [传染病];

学科分类号：

100401 ;

摘要：

Background: In 2006, the Senegalese National Malaria Control Programme recommended artemisinin-based combination therapy (ACT) as the first-line treatment for uncomplicated malaria. Since the introduction of ACT, there have been very few reports on the level of Plasmodium falciparum resistance to anti-malarial drugs. An ex vivo susceptibility study was conducted on local isolates obtained from the Hopital Principal de Dakar (Dakar, Senegal) from November 2013 to January 2014. Methods: Eighteen P. falciparum isolates were sussessfully assessed for ex vivo susceptibility to chloroquine (CQ), quinine (QN), monodesethylamodiaquine (MDAQ), the active metabolite of amodiaquine, mefloquine (MQ), lumefantrine (LMF), artesunate (AS), dihydroartemisinin (DHA), the active metabolite of artemisinin derivatives, pyronaridine (PND), piperaquine (PPQ), and, Proveblue (PVB), a methylene blue preparation, using the HRP2-based ELISA test. Results: The prevalence of isolates with reduced susceptibility was 55.6% for MQ, 50% for CQ, 5.6% for QN and MDAQ, and 0% for DHA, AS and LMF. The mean IC50 for PND, PPQ and PVB were 5.8 nM, 32.2 nM and 5.3 nM, respectively. Conclusions: The prevalence of isolates with a reduced susceptibility to MQ remains high and stable in Dakar. Since 2004, the prevalence of CQ resistance decreased, but rebounded in 2013 in Dakar. PND, PPQ and PVB showed high in vitro activity in P. falciparum parasites from Dakar.

引用

页数：7

共 58 条

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