Toward the Second Generation of Optogenetic Tools

被引：79

作者：

Knoepfel, Thomas ^{[1
]}

Lin, Michael Z. ^{[2
,3
]}

Levskaya, Anselm ^{[4
]}

Tian, Lin ^{[5
]}

Lin, John Y. ^{[6
]}

Boyden, Edward S. ^{[7
,8
]}

机构：

[1] RIKEN Brain Sci Inst, Lab Neuronal Circuit Dynam, Wako, Saitama 3510198, Japan

[2] Stanford Univ, Dept Pediat, Stanford, CA 94305 USA

[3] Stanford Univ, Dept Bioengn, Stanford, CA 94305 USA

[4] Univ Calif San Francisco, Dept Cellular & Mol Pharmacol, San Francisco, CA 94143 USA

[5] Howard Hughes Med Inst, Ashburn, VA 20147 USA

[6] Univ Calif, Howard Hughes Med Inst, Palade Lab, La Jolla, CA 92093 USA

[7] MIT, Media Lab, Cambridge, MA 02139 USA

[8] MIT, McGovern Inst, Cambridge, MA 02139 USA

来源：

JOURNAL OF NEUROSCIENCE | 2010年 / 30卷 / 45期

关键词：

GREEN FLUORESCENT PROTEINS; ENCODED CALCIUM INDICATOR; IN-VIVO; CA2+; CHANNELRHODOPSIN-2; SINGLE; PROBE; INTERROGATION; HALORHODOPSIN; ACTIVATION;

D O I：

10.1523/JNEUROSCI.4190-10.2010

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

This mini-symposium aims to provide an integrated perspective on recent developments in optogenetics. Research in this emerging field combines optical methods with targeted expression of genetically encoded, protein-based probes to achieve experimental manipulation and measurement of neural systems with superior temporal and spatial resolution. The essential components of the optogenetic toolbox consist of two kinds of molecular devices: actuators and reporters, which respectively enable light-mediated control or monitoring of molecular processes. The first generation of genetically encoded calcium reporters, fluorescent proteins, and neural activators has already had a great impact on neuroscience. Now, a second generation of voltage reporters, neural silencers, and functionally extended fluorescent proteins hold great promise for continuing this revolution. In this review, we will evaluate and highlight the limitations of presently available optogenic tools and discuss where these technologies and their applications are headed in the future.

引用

页码：14998 / 15004

页数：7

共 60 条

[1] Exploration of new chromophore structures leads to the identification of improved blue fluorescent proteins [J].

Ai, Hui-wang ;

Shaner, Nathan C. ;

Cheng, Zihao ;

Tsien, Roger Y. ;

Campbell, Robert E. .

BIOCHEMISTRY, 2007, 46 (20) :5904-5910

[2]

Akemann W, 2010, NAT METHODS, V7, P643, DOI [10.1038/NMETH.1479, 10.1038/nmeth.1479]

[3] A genetically targetable fluorescent probe of channel gating with rapid kinetics [J].

Ataka, K ;

Pieribone, VA .

BIOPHYSICAL JOURNAL, 2002, 82 (01) :509-516

[4] Circular permutation and receptor insertion within green fluorescent proteins [J].

Baird, GS ;

Zacharias, DA ;

Tsien, RY .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1999, 96 (20) :11241-11246

[5] Genetically encoded fluorescent sensors of membrane potential [J].

Baker, B. J. ;

Mutoh, H. ;

Dimitrov, D. ;

Akemann, W. ;

Perron, A. ;

Iwamoto, Y. ;

Jin, L. ;

Cohen, L. B. ;

Isacoff, E. Y. ;

Pieribone, V. A. ;

Hughes, T. ;

Knopfel, T. .

BRAIN CELL BIOLOGY, 2008, 36 (1-4) :53-67

[6] Structural Guidance of the Photocycle of Channelrhodopsin-2 by an Interhelical Hydrogen Bond [J].

Bamann, Christian ;

Gueta, Ronnie ;

Kleinlogel, Sonja ;

Nagel, Georg ;

Bamberg, Ernst .

BIOCHEMISTRY, 2010, 49 (02) :267-278

[7]

Boldogköi Z, 2009, NAT METHODS, V6, P127, DOI [10.1038/NMETH.1292, 10.1038/nmeth.1292]

[8] Millisecond-timescale, genetically targeted optical control of neural activity [J].

Boyden, ES ;

Zhang, F ;

Bamberg, E ;

Nagel, G ;

Deisseroth, K .

NATURE NEUROSCIENCE, 2005, 8 (09) :1263-1268

[9] High-performance genetically targetable optical neural silencing by light-driven proton pumps [J].

Chow, Brian Y. ;

Han, Xue ;

Dobry, Allison S. ;

Qian, Xiaofeng ;

Chuong, Amy S. ;

Li, Mingjie ;

Henninger, Michael A. ;

Belfort, Gabriel M. ;

Lin, Yingxi ;

Monahan, Patrick E. ;

Boyden, Edward S. .

NATURE, 2010, 463 (7277) :98-102

[10] Fluorescent Proteins and Their Applications in Imaging Living Cells and Tissues [J].

Chudakov, Dmitriy M. ;

Matz, Mikhail V. ;

Lukyanov, Sergey ;

Lukyanov, Konstantin A. .

PHYSIOLOGICAL REVIEWS, 2010, 90 (03) :1103-1163

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