Binding properties of 2-acetylnaphthalene with hydroxypropyl cyclodextrins from fluorescence quenching experiments

The quenching of 2-acetyinaphthalene (2-AN) fluorescence by hydroxypropyl cyclodextrins (HP-CD) has been analyzed using modified Stern-Volmer plots to obtain binding constants as a function of temperature for 2-AN:HP-CD complexes. The HP-CDs were commercially available and contained 4-7 HP groups per CD molecule for α-CD, β-CD, and γ-CD. HP substitution causes a 12 to over 40% increase in binding constant (K-ave) for 2-AN compared to that for unsubstituted CDs, although the K-ave value is not strongly dependent on the extent of HP substitution for β-CD. No evidence of formation of a 2:2 complex, such as that observed with 2-AN and γ-CD, is observed with 2-AN and HP-γ-CD. Thermodynamic parameters (&UDelta; H° and &UDelta; S°) suggest that the increase in K-ave with HP substitution is due to an enlarged binding site for the HP-CDs that allows greater motional freedom for 2-AN. Comparison is made to the binding of 2-methyinaphthoate (2-MN) to CDs and HP-CDs, and the larger K-ave values for 2-MN over 2-AN are attributed to greater dispersion forces for 2-MN complex formation.