Insulator and silencer sequences in the imprinted region of human chromosome 11p15.5

被引：52

作者：

Du, MJ

Beatty, LG

Zhou, WJ

Lew, J

Schoenherr, C

Weksberg, R

Sadowski, PD ^{[1
]}

机构：

[1] Univ Toronto, Dept Mol & Med Genet, Toronto, ON M5S 1A8, Canada

[2] Univ Illinois, Dept Cell & Struct Biol, Urbana, IL 61801 USA

[3] Univ Toronto, Dept Paediat, Toronto, ON M5G 1X8, Canada

[4] Hosp Sick Children, Dept Clin & Metab Genet, Toronto, ON M5G 1X8, Canada

来源：

HUMAN MOLECULAR GENETICS | 2003年 / 12卷 / 15期

关键词：

D O I：

10.1093/hmg/ddg194

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The imprinting of the genes on human chromosome 11p15.5 is thought to be controlled by two imprinting control regions located in two differentially methylated CpG islands upstream of the H19 gene (H19 DMR) and in intron 10 of the KCNQ1 gene (KvDMR). We have examined sequences in the human 11p15.5 genomic imprinted region for the presence of insulators and silencers using a position- and enhancer-dependent stable transfection assay. We have confirmed the existence of insulators in H19 DMR and discovered two novel insulators in the IGF2 gene. We have also found two novel silencer sequences; one is located in KvDMR, a region that is thought to contain the promoter for the KCNQ1OT1 transcript, and another is in the CDKN1C gene. We have demonstrated binding of CTCF protein in vitro to all the insulator and silencer sequences that we have detected. We discuss the differences in the regulation of imprinting controlled by the two imprinting control regions in chromosome 11p.

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页码：1927 / 1939

页数：13

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