Survey of severe fever with thrombocytopenia syndrome virus in wild boar in the Republic of Korea

被引：11

作者：

Rim, Ji-Min ^{[1
,2
]}

Han, Sun-Woo ^{[1
,2
]}

Cho, Yoon-Kyoung ^{[1
,2
]}

Kang, Jun-Gu ^{[3
]}

Choi, Kyoung-Seong ^{[4
]}

Jeong, Hyesung ^{[5
]}

Son, Kidong ^{[5
]}

Kim, Jisoo ^{[5
]}

Choi, Yongjun ^{[5
]}

Kim, Won-Meong ^{[5
]}

Cho, Nam-Hyuk ^{[6
]}

Chae, Joon-Seok ^{[1
,2
]}

机构：

[1] Seoul Natl Univ, Lab Vet Internal Med, BK21 PLUS Program Creat Vet Sci Res, Res Inst Vet Sci, 1 Gwanak Ro, Seoul 08826, South Korea

[2] Seoul Natl Univ, Coll Vet Med, 1 Gwanak Ro, Seoul 08826, South Korea

[3] Jeonbuk Natl Univ, Korea Zoonosis Res Inst, Iksan 54531, South Korea

[4] Kyungpook Natl Univ, Coll Ecol & Environm Sci, Sangju 37224, South Korea

[5] Natl Inst Wildlife Dis Control & Prevent, 1 Songam Gil, Gwangju 62407, South Korea

[6] Seoul Natl Univ, Dept Microbiol & Immunol, Coll Med, Seoul 03080, South Korea

来源：

TICKS AND TICK-BORNE DISEASES | 2021年 / 12卷 / 06期

关键词：

Dabie bandavirus; Severe fever with thrombocytopenia syndrome; Tick-borne diseases; Wild boar; Antigen; Antibody; MOLECULAR-DETECTION; SHANDONG PROVINCE; SOUTH-KOREA; INFECTION; ANIMALS; ANAPLASMA; CATS;

D O I：

10.1016/j.ttbdis.2021.101813

中图分类号：

R51 [传染病];

学科分类号：

100401 ;

摘要：

Severe fever with thmmbocytopenia syndrome (SFTS) is caused by Dabie bandavirus that belongs to the genus Bandavirus in the family Phenuiviridae and order Bunyavirales and is transmitted by hard ticks. It has been detected in several tick species, various animals, and humans. The purpose of this study was to detect SFTS virus (SFTSV) antigen and antibody in wild boar in the Republic of Korea (ROK). A total of 768 sera samples were collected from wild boar in the ROK between January and December 2019. Viral RNA was extracted from sera using viral RNA extraction kit, and one-step RT-nested polymerase chain reaction (PCR) was performed to amplify the S segment of the SFTSV. The sequencing data were analyzed using Chromas and aligned using Clustal X. The phylogenetic tree was constructed using the maximum-likelihood method using MEGA7. In addition, wild boar sera were tested for IgG antibodies against SFTSV by enzyme-linked immunosorbent assay (ELISA) and immunofluorescence assay (IFA). Of a total of 768 sera samples, 40 (5.2%) were positive for SFTSV by RT-PCR targeting the S segment. Two hundred twenty-one (28.8%) and 159 (20.7%) of 768 sera samples were sempositive by ELISA and IFA, respectively. Based on both ELISA and IFA tests of the same samples, 110 (14.3%) wild boar sera samples were positive for SFTSV antibodies. Of a total of 40 positive serum samples by RT-PCR, 33 (82.5%) and 7 (17.5%) sera were classified as the genotype B-3 and D, respectively, by sequence analysis,. These results provide useful information that demonstrates the detection of antigen and antibody in wild boar sera samples for every month of a certain year throughout the ROK.

引用

页数：8

共 41 条

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