Matrigel coated polydimethylsiloxane based microfluidic devices for studying metastatic and non-metastatic cancer cell invasion and migration

被引：49

作者：

Chaw, K. C.

Manimaran, M.

Tay, F. E. H.

Swaminathan, S.

机构：

[1] Inst Med Biol, Singapore 138668, Singapore

[2] NUS, Grad Sch Integrat Sci & Engn, Singapore 117598, Singapore

[3] Dept Engn Mech, Singapore 119260, Singapore

[4] NUS, Oncol Res Inst, Singapore 117597, Singapore

[5] NUS, Dept Physiol, Singapore 117597, Singapore

来源：

BIOMEDICAL MICRODEVICES | 2007年 / 9卷 / 04期

关键词：

microfluidic devices; matrigel coating; extracellular matrices (ECM); cell migration; cell invasion;

D O I：

10.1007/s10544-007-9071-5

中图分类号：

R318 [生物医学工程];

学科分类号：

0831 ;

摘要：

Three-dimensional (3-D) extracellular matrices (ECM) allow complex biochemical and biophysical interactions between cells and matrices. Unlike 2-D systems, 3-D models provide a better representation of the micro and local environments in living tissues for facilitating the physiological study of cell migration. Here, we report a microfluidic device based on olydimethylsiloxane (PDMS) for monitoring 3-D cell migration across ECM-coated microgaps with real-time light microscopy. We tracked the migration of the invasive MDA-MB-231 (mammary carcinoma) cells and mapped out their migration paths. It enabled us to quantify the percentage of migrated cells as well as migration information of individual cells. This wide spectrum of data acquisition is vital for elucidating the migration capabilities of different type of cells and to understand the basic mechanism involved in cancer metastasis.

引用

页码：597 / 602

页数：6

共 31 条

[1] The "chemoinvasion assay": a tool to study tumor and endothelial cell invasion of basement membranes [J].