Genome-Wide Analysis and Expression Profiling of Glutathione Reductase Gene Family in Oat (Avena sativa) Indicate Their Responses to Abiotic Stress during Seed Imbibition

被引:7
作者
Sun, Ming [1 ]
Sun, Shoujiang [1 ]
Jia, Zhicheng [1 ]
Ma, Wen [1 ]
Mao, Chunli [1 ]
Ou, Chengming [1 ]
Wang, Juan [1 ]
Zhang, Han [1 ]
Hong, Liu [1 ]
Li, Manli [1 ]
Jia, Shangang [1 ]
Mao, Peisheng [1 ]
机构
[1] China Agr Univ, Coll Grassland Sci & Technol, Forage Seed Lab, Beijing 100193, Peoples R China
关键词
A; sativa; glutathione reductase; abiotic stress; seed imbibition; qPCR; SALT STRESS; LEAF SENESCENCE; BRASSICA-JUNCEA; GERMINATION; L; ASCORBATE; CLONING; ROS; OVEREXPRESSION; IDENTIFICATION;
D O I
10.3390/ijms231911650
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Abiotic stress disturbs plant cellular redox homeostasis, inhibiting seed germination and plant growth. This is a crucial limitation to crop yield. Glutathione reductase (GR) is an important component of the ascorbate-glutathione (AsA-GSH) cycle which is involved in multiple plant metabolic processes. In the present study, GRs in A. sativa (AsGRs) were selected to explore their molecular characterization, phylogenetic relationship, and RNA expression changes during seed imbibition under abiotic stress. Seven AsGR genes were identified and mapped on six chromosomes of A, C, and D subgenomes. Phylogenetic analysis and subcellular localization of AsGR proteins divided them into two sub-families, AsGR1 and AsGR2, which were predicted to be mainly located in cytoplasm, mitochondrion, and chloroplast. Cis-elements relevant to stress and hormone responses are distributed in promoter regions of AsGRs. Tissue-specific expression profiling showed that AsGR1 genes were highly expressed in roots, leaves, and seeds, while AsGR2 genes were highly expressed in leaves and seeds. Both AsGR1 and AsGR2 genes showed a decreasing-increasing expression trend during seed germination under non-stress conditions. In addition, their responses to drought, salt, cold, copper, H2O2, and ageing treatments were quite different during seed imbibition. Among the seven AsGR genes, AsGR1-A, AsGR1-C, AsGR2-A, and AsGR2-D responded more significantly, especially under drought, ageing, and H2O2 stress. This study has laid the ground for the functional characterization of GR and the improvement of oat stress tolerance and seed vigor.
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页数:19
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