Gelsemium elegans cyclic peptide induces human cervical carcinoma cells apoptosis through intrinsic and extrinsic pathways

被引：1

作者：

Liu, Jia ^{[1
]}

Liu, Fangting ^{[1
]}

Liu, Pingping ^{[1
]}

Xu, Hai ^{[1
]}

Tang, Longguo ^{[1
]}

Han, Xiuxia ^{[1
]}

Zheng, Man ^{[1
]}

Ren, Yuebing ^{[1
]}

机构：

[1] Dongying Peoples Hosp, Dongying 257091, Shandong, Peoples R China

来源：

JOURNAL OF PEPTIDE SCIENCE | 2022年 / 28卷 / 09期

关键词：

cyclic peptide; Gelsemium elegans; human cervical carcinoma; intrinsic pathway; ACTIVATION; ALKALOIDS; CASPASE-3; DEATH;

D O I：

10.1002/psc.3410

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Four novel Gelsemium elegans cyclic peptides (GEPs) were isolated in an antihuman cervical carcinoma activity tracking method, and their amino acid sequences were identified. The GEP-1 cyclic-(Trp-Leu-His-Val)-peptide inhibited HeLa cell proliferation in a dose- and time-dependent manner. GEP-1 induced intracellular reactive oxygen species (ROS) overproduction and induced HeLa cells apoptosis in a caspase-dependent manner. GEP-1 also induced collapse of the mitochondrial membrane potential and promoted the mitochondrial release of cytochrome c (cyt c), apoptosis-inducing factor (AIF), and endonuclease G (Endo G) in HeLa cells. Furthermore, GEP-1 triggered the extrinsic death receptor-dependent pathway, which was characterized by activating Fas and FADD. Notably, GEP-1 is a potential antihuman cervical carcinoma peptide.

引用

页数：9

共 20 条

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