The tumor-suppressive function of miR-1296-5p by targeting EGFR and CDK6 in gastric cancer

被引：21
作者：
Jia, Yan [1 ,2 ]
Zhao, Lian-Mei [1 ]
Bai, Han-Yu [1 ]
Zhang, Cong [1 ]
Dai, Su-Li [1 ]
Lv, Hui-Lai [3 ]
Shan, Bao-En [1 ]
机构：
[1] Hebei Med Univ, Res Ctr, Hosp 4, Shijiazhuang 050011, Hebei, Peoples R China
[2] Hebei Med Univ, Dept Cardiol, Hosp 2, Shijiazhuang, Hebei, Peoples R China
[3] Hebei Med Univ, Dept Thorac Surg, Hosp 4, Shijiazhuang 050011, Hebei, Peoples R China
来源：
BIOSCIENCE REPORTS | 2019年 / 39卷
关键词：
KINASE; 6; EXPRESSION; CELL-PROLIFERATION; GROWTH; PROGRESSION; MICRORNA-1296; PATHWAYS; THERAPY; FAMILY; IMPACT;
D O I：
10.1042/BSR20181556
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
We aimed to confirm the role of miR-1296-5p in gastric cancer and to identify its target genes. The expression of miR-1296-5p was measured in gastric cancer tissues and cell lines. The function of miR-1296-5p was examined by the overexpression and inhibition of its expression in typical gastric cell lines as well as SGC-7901 and MGC-803 cells. The targets of miR-1296-5p were identified by a luciferase activity assay. We found that miR-1296-5p was down-regulated in gastric cancer tissue and cell lines, and low expression levels of miR-1296-5p were associated with advanced clinical stage. Moreover, miR-1296-5p inhibited cell proliferation, migration, and invasion in SGC-7901 and MGC-803 cells. Then, we identified CDK6 and EGFR as novel targets of miR-1296-5p by a luciferase activity assay. Furthermore, the overexpression of miR-1296-5p suppressed the expression of CDK6 and EGFR. Our results indicated a tumor-suppressive role of miR-1296-5p through the translational repression of oncogenic CDK6 and EGFR in gastric cancer.
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页数：17
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