Genomic identity of pyelonephritogenic Escherichia coli isolated from blood, urine and faeces of children with urosepsis

被引:27
作者
Jantunen, ME
Saxén, H
Lukinmaa, S
Ala-Houhala, M
Siitonen, A
机构
[1] Univ Helsinki, Hosp Children & Adolescents, FIN-00290 Helsinki, Finland
[2] Natl Publ Hlth Inst, KTL, Lab Enter Pathogens, FIN-00280 Helsinki, Finland
[3] Tampere Univ Hosp, Dept Pediat, FIN-33521 Tampere, Finland
关键词
D O I
10.1099/0022-1317-50-7-650
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Chromosomal genotypes of Escherichia coli isolates from blood, urine and faeces of infants with urosepsis were studied to find possible clonality of the isolates. The isolates were analysed by PCR for class I, II and III alleles of the pyelonephritis-associated adhesin gene papG, The macrorestriction profiles of the papG-positive isolates were analysed by pulsed-field gel electrophoresis and their O serogroups were determined. Genetically identical E. coli isolates from the blood, urine and faeces of the same infant were found in 8 of 10 infants. This finding confirmed the results of previous phenotypic studies that the reservoir of pyelonephritogenic E. coli is indeed the colon.
引用
收藏
页码:650 / 652
页数:3
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