Small-angle X-ray scattering method to characterize molecular interactions: Proof of concept

被引：29

作者：

Allec, Nicholas ^{[1
]}

Choi, Mina ^{[1
,2
]}

Yesupriya, Nikhil ^{[1
]}

Szychowski, Brian ^{[3
]}

White, Michael R. ^{[3
]}

Kann, Maricel G. ^{[4
]}

Garcin, Elsa D. ^{[3
]}

Daniel, Marie-Christine ^{[3
]}

Badano, Aldo ^{[1
]}

机构：

[1] US FDA, Div Imaging Diagnost & Software Reliabil, Off Sci & Engn Labs, Ctr Devices & Radiol Hlth, Silver Spring, MD 20993 USA

[2] Univ Maryland, Fischell Dept Bioengn, College Pk, MD 20742 USA

[3] Univ Maryland, Dept Chem & Biochem, College Pk, MD USA

[4] Univ Maryland, Dept Biol Sci, College Pk, MD USA

来源：

SCIENTIFIC REPORTS | 2015年 / 5卷

基金：

美国国家科学基金会;

关键词：

PROTEIN-PROTEIN INTERACTIONS; GOLD NANOPARTICLES; BIOLOGICAL MACROMOLECULES; STRUCTURAL-CHANGES; SIZE; DNA; DISTANCE; SYSTEM;

D O I：

10.1038/srep12085

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Characterizing biomolecular interactions is crucial to the understanding of biological processes. Existing characterization methods have low spatial resolution, poor specificity, and some lack the capability for deep tissue imaging. We describe a novel technique that relies on small-angle X-ray scattering signatures from high-contrast molecular probes that correlate with the presence of biomolecular interactions. We describe a proof-of-concept study that uses a model system consisting of mixtures of monomer solutions of gold nanoparticles (GNPs) as the non-interacting species and solutions of GNP dimers linked with an organic molecule (dimethyl suberimidate) as the interacting species. We report estimates of the interaction fraction obtained with the proposed small-angle X-ray scattering characterization method exhibiting strong correlation with the known relative concentration of interacting and non-interacting species.

引用

页数：12

共 54 条

[11] Toxicity assessment of nanomaterials: methods and challenges [J].

Dhawan, Alok ;

Sharma, Vyom .

ANALYTICAL AND BIOANALYTICAL CHEMISTRY, 2010, 398 (02) :589-605

[12] Bioluminescence resonance energy transfer (BRET) imaging of protein-protein interactions within deep tissues of living subjects [J].

Dragulescu-Andrasi, Anca ;

Chan, Carmel T. ;

De, Abhijit ;

Massoud, Tarik F. ;

Gambhir, Sanjiv S. .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2011, 108 (29) :12060-12065

[13] PEGylated Gold Nanoparticles Conjugated to Monoclonal F19 Antibodies as Targeted Labeling Agents for Human Pancreatic Carcinoma Tissue [J].

Eck, Wolfgang ;

Craig, Gary ;

Sigdel, Aruna ;

Ritter, Gerd ;

Old, Lloyd J. ;

Tang, Laura ;

Brennan, Murray F. ;

Allen, Peter J. ;

Mason, Michael D. .

ACS NANO, 2008, 2 (11) :2263-2272

[14]

Feigin L. A., 1987, Structure Analysis by SmallAngle XRay and Neutron Scattering

[15] USAXS and SAXS from cancer-bearing breast tissue samples [J].

Fernandez, M. ;

Suhonen, H. ;

Keyrilainen, J. ;

Bravin, A. ;

Fiedler, S. ;

Karjalainen-Lindsberg, M. -L. ;

Leidenius, A. ;

von Smitten, K. ;

Suortti, P. .

EUROPEAN JOURNAL OF RADIOLOGY, 2008, 68 (03) :S89-S94

[16] DAMMIF, a program for rapid ab-initio shape determination in small-angle scattering [J].

Franke, Daniel ;

Svergun, Dmitri I. .

JOURNAL OF APPLIED CRYSTALLOGRAPHY, 2009, 42 :342-346

[17] CONTROLLED NUCLEATION FOR REGULATION OF PARTICLE-SIZE IN MONODISPERSE GOLD SUSPENSIONS [J].

FRENS, G .

NATURE-PHYSICAL SCIENCE, 1973, 241 (105) :20-22

[18] NEW METHOD FOR EVALUATION OF SMALL-ANGLE SCATTERING DATA [J].

GLATTER, O .

JOURNAL OF APPLIED CRYSTALLOGRAPHY, 1977, 10 (OCT1) :415-421

[19]

Glatter O, 1982, Small angle x-ray scattering

[20] Controlled Formation of Gold Nanoparticle Dimers Using Multivalent Thiol Ligands [J].

Hofmann, Andreas ;

Schmiel, Peter ;

Stein, Benjamin ;

Graf, Christina .

LANGMUIR, 2011, 27 (24) :15165-15175

← 1 2 3 4 5 6 →