Characterization of human rabies virus vaccine strain in China

被引：25

作者：

Du, Jialiang ^{[1
,2
]}

Zhang, Qiang ^{[1
,2
]}

Tang, Qing ^{[1
,2
]}

Li, Hao ^{[1
,2
]}

Tao, Xiaoyan ^{[1
,2
]}

Morimoto, Kinjiro ^{[3
]}

Nadin-Davis, Susan A. ^{[4
]}

Liang, Guodong ^{[1
,2
]}

机构：

[1] Chinese Ctr Dis Control & Prevent, IVDC, China CDC, Dept Viral Encephalitis,Inst Viral Dis Control &, Beijing 100052, Peoples R China

[2] State Key Lab Infect Dis Prevent & Control SKLID, Beijing 100052, Peoples R China

[3] Natl Inst Infect Dis, Dept Virol 1, Shinjuku Ku, Tokyo 1628640, Japan

[4] Canadian Food Inspect Agcy, Ottawa Lab Fallowfield, Rabies Ctr Expertise, Nepean, ON K2H 8P9, Canada

来源：

VIRUS RESEARCH | 2008年 / 135卷 / 02期

基金：

中国国家自然科学基金;

关键词：

characterization; CTN181; aG; rabies virus; China;

D O I：

10.1016/j.virusres.2008.04.002

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Human rabies virus vaccine strain CFN181 from China was sequenced. The overall length of the genome was 11,923 nucleotides (nt), comprising a leader sequence of 58 nt, nucleoprotein (N) gene of 1353 nt, phosphoprotein (P) gene of 894 nt, matrix protein (M) gene of 609 nt, glycoprotein (G) gene of 1575 nt, RNA-dependent RNA polymerase (RdRp, L) gene of 6387 nt, and a trailer region of 70 nt. The five monocistrons are separated by intergenic regions (IGRs) of 2, 5, 5 and 24 nucleotides (nt), respectively. Two obvious differences between CTN181 and the other rabies virus vaccine strains were (1) the putative stop/polyradenylation signal of the G gene has only one poly (A) tract for CTN 181, and (2) the start of the open reading frame for L has two repeats of ATG for CTN181. Both were similar to the SHBRV-18 (silver-haired bat-associated RV strain 18) strain. In addition, some mutations and new functional regions were discovered that are presumed crucial to the function of leader region and L protein. There is an equal role for all five genes in the phylogenetics of rabies virus. (C) 2008 Elsevier B.V. All rights reserved.

引用

页码：260 / 266

页数：7

共 48 条

[1] De novo synthesis of RNA by the dengue virus RNA-dependent RNA polymerase exhibits temperature dependence at the initiation but not elongation phase [J].

Ackermann, M ;

Padmanabhan, R .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2001, 276 (43) :39926-39937

[2]

ASHIM KG, 2000, J VIROL, V74, P91

[3] GENE-EXPRESSION OF VESICULAR STOMATITIS-VIRUS GENOME RNA [J].

BANERJEE, AK ;

BARIK, S .

VIROLOGY, 1992, 188 (02) :417-428

[4] N-PROTEIN OF VESICULAR STOMATITIS-VIRUS SELECTIVELY ENCAPSIDATES LEADER RNA INVITRO [J].

BLUMBERG, BM ;

GIORGI, C ;

KOLAKOFSKY, D .

CELL, 1983, 32 (02) :559-567

[5] Phylogenetic relationships among rhabdoviruses inferred using the L polymerase gene [J].

Bourhy, H ;

Cowley, JA ;

Larrous, F ;

Holmes, EC ;

Walker, PJ .

JOURNAL OF GENERAL VIROLOGY, 2005, 86 :2849-2858

[6] SEQUENCE AND SECONDARY STRUCTURE-ANALYSIS OF THE 5'-TERMINAL REGION OF FLAVIVIRUS GENOME RNA [J].

BRINTON, MA ;

DISPOTO, JH .

VIROLOGY, 1988, 162 (02) :290-299

[7] Mapping the interacting domains between the rabies virus polymerase and phosphoprotein [J].

Chenik, M ;

Schnell, M ;

Conzelmann, KK ;

Blondel, D .

JOURNAL OF VIROLOGY, 1998, 72 (03) :1925-1930

[8] Identification of viral genomic elements responsible for rabies virus neuroinvasiveness [J].

Faber, M ;

Pulmanausahakul, R ;

Nagao, K ;

Prosniak, M ;

Rice, AB ;

Koprowski, H ;

Schnell, MJ ;

Dietzschold, B .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2004, 101 (46) :16328-16332

[9] A single immunization with a rhabdovirus-based vector expressing severe acute respiratory syndrome coronavirus (SARS-CoV) S protein results in the production of high levels of SARS-CoV-neutralizing antibodies [J].

Faber, M ;

Lamirande, EW ;

Roberts, A ;

Rice, AB ;

Koprowski, H ;

Dietzschold, B ;

Schnell, MJ .

JOURNAL OF GENERAL VIROLOGY, 2005, 86 :1435-1440

[10] Differential transcription attenuation of rabies virus genes by intergenic regions: Generation of recombinant viruses overexpressing the polymerase gene [J].

Finke, S ;

Cox, JH ;

Conzelmann, KK .

JOURNAL OF VIROLOGY, 2000, 74 (16) :7261-7269

← 1 2 3 4 5 →