The expression of neuroglobin in astrocytoma

被引：20

作者：

Qin, Haojie ^{[1
,2
]}

Guo, Yadong ^{[3
]}

Zhang, Changquan ^{[1
]}

Zhang, Lin ^{[1
]}

Li, Mingyuan ^{[1
]}

Guan, Peng ^{[1
]}

机构：

[1] Sichuan Univ, Dept Forens Pathol, W China Sch Preclin & Forens Med, Chengdu 610041, Peoples R China

[2] Henan Univ Sci & Technol, Sch Forens Med, Luoyang 471003, Peoples R China

[3] Cent S Univ, Dept Forens Sci, Sch Basic Med Sci, Changsha 410013, Hunan, Peoples R China

来源：

BRAIN TUMOR PATHOLOGY | 2012年 / 29卷 / 01期

基金：

中国国家自然科学基金;

关键词：

Neuroglobin; Astrocytoma; RNA expression; Protein expression; HYPOXIA-INDUCIBLE FACTOR-1; GLOBIN FAMILY; MESSENGER-RNA; CYTOCHROME-C; IN-VIVO; BRAIN; GLIOMA; OXYGEN; ANGIOGENESIS; LOCALIZATION;

D O I：

10.1007/s10014-011-0066-9

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

Neuroglobin (NGB) is a characterized heme globin that is widely expressed in the vertebrate central and peripheral nervous system as well as retina and endocrine tissues. However, to date, it is not determined whether functional NGB is expressed in cells of glia origin. In this study, we aimed to explore the detailed expression of NGB in a rat astrocytoma cell line (C6) and human astrocytoma cell line (U251) by reverse transcription-polymerase chain reaction, immunofluorescence, and Western blotting, and to detect the expression of NGB in human astrocytoma tissues by an immunohistochemical method. We found that NGB was present in a rat astrocytoma cell line (C6), human astrocytoma cell line (U251), and human astrocytoma tissues. The expression and potential roles of NGB in astrocytomas may provide insight into the mechanisms of tumor cells to adapt and survive in hypoxic microenvironments and also represent a novel therapeutic approach to astrocytomas.

引用

页码：10 / 16

页数：7

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