Diurnal and circadian variation of protein kinase C immunoreactivity in the rat retina

被引:35
作者
Gabriel, R
Lesauter, J
Silver, R
Garcia-España, A
Witkovsky, P
机构
[1] NYU, Sch Med, Dept Ophthalmol, New York, NY 10016 USA
[2] Univ Pecs, Dept Gen Zool & Neurobiol, H-7604 Pecs, Hungary
[3] Columbia Univ Barnard Coll, Dept Psychol, New York, NY 10027 USA
[4] Columbia Univ, Dept Psychol, New York, NY 10027 USA
[5] Columbia Univ Coll Phys & Surg, Dept Anat & Cell Biol, New York, NY 10032 USA
[6] NYU, Sch Med, Dept Psychiat, New York, NY 10016 USA
[7] NYU, Sch Med, Dept Physiol & Neurosci, New York, NY 10016 USA
关键词
rod bipolar cell; amacrine cell; immunocytochemistry; kinase;
D O I
10.1002/cne.1338
中图分类号
Q189 [神经科学];
学科分类号
071006 ;
摘要
We studied the dependence of the expression of protein kinase C immunoreactivity (PKC-IR) in the rat retina on the light:dark (LD) cycle and on circadian rhythmicity in complete darkness (DD). Two anti-PKC alpha antibodies were employed: One, which we call PKC alpha beta recognized the hinge region; the other, here termed PKC alpha, recognized the regulatory region of the molecule. Western blots showed that both anti-PKC antibodies stained an identical single band at approximately 80 kD. The retinal neurons showing PKC-IR were rod bipolar cells and a variety of amacrine neurons. After 3 weeks on an LD cycle, PKC alpha beta -IR in both rod bipolar and certain amacrine cells manifested a clear rhythm with a peak at zeitgeber time (ZT) of 06-10 hours and a minimum at ZT 18. No rhythm in total PKC-IR was observed when using the PKC alpha antibody, but, at ZT 06-10 hours, rod bipolar axon terminals showed increased immunostaining. After 48 hours in DD, with either antibody, rod bipolar cells showed increased PKC-IR. The PKC alpha antibody alone revealed that, after 48 hours, AII amacrine neurons, which lacked PKC-IR in an LD cycle, manifested marked PKC-IR, which became stronger after 72 hours. Light administered early in the dark period greatly increased PKC alpha beta -IR in rod bipolar and some amacrine neurons. Our data indicate that light and darkness exert a strong regulatory influence on PKC synthesis, activation, and transport in retinal neurons. (C) 2001 Wiley-Liss, Inc.
引用
收藏
页码:140 / 150
页数:11
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