Protective Effect of Ocotillol, the Derivate of Ocotillol-Type Saponins in Panax Genus, against Acetic Acid-Induced Gastric Ulcer in Rats Based on Untargeted Metabolomics

被引：15
作者：
Wang, Cuizhu [1 ,2 ]
Yuan, Yuze [1 ]
Pan, He [1 ]
Hsu, Alan Chen-Yu [3 ]
Chen, Jinluan [4 ]
Liu, Jinping [2 ]
Li, Pingya [2 ]
Wang, Fang [1 ]
机构：
[1] Jilin Univ, Coll Basic Med Sci, Dept Pathogen Biol, Changchun 130021, Peoples R China
[2] Jilin Univ, Sch Pharmaceut Sci, Fujin Rd 1266, Changchun 130021, Peoples R China
[3] Univ Newcastle, Fac Hlth & Med, Prior Res Ctr Hlth Lungs, Newcastle, NSW 2305, Australia
[4] Univ Med Ctr Rotterdam, Dept Internal Med, Erasmus MC, NL-3031 RM Rotterdam, Netherlands
来源：
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | 2020年 / 21卷 / 07期
关键词：
Ocotillol; gastric ulcer; metabolomics; UPLC-QTOF-MS; ALZHEIMERS-DISEASE; LINOLEIC-ACID; GINSENG; INHIBITORS; QUERCETIN; MECHANISM; RELEASE; DESIGN; MICE;
D O I：
10.3390/ijms21072577
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Gastric ulcer (GU), a prevalent digestive disease, has a high incidence and is seriously harmful to human health. Finding a natural drug with a gastroprotective effect is needed. Ocotillol, the derivate of ocotillol-type saponins in the Panax genus, possesses good anti-inflammatory activity. The study aimed to investigate the gastroprotective effect of ocotillol on acetic acid-induced GU rats. The serum levels of endothelin-1 (ET-1) and nitric oxide (NO), the gastric mucosa levels of epidermal growth factor, superoxide dismutase and NO were assessed. Hematoxylin and eosin staining of gastric mucosa for pathological changes and immunohistochemical staining of ET-1, epidermal growth factor receptors and inducible nitric oxide synthase were evaluated. A UPLC-QTOF-MS-based serum metabolomics approach was applied to explore the latent mechanism. A total of 21 potential metabolites involved in 7 metabolic pathways were identified. The study helps us to understand the pathogenesis of GU and to provide a potential natural anti-ulcer agent.
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页数：19
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