Column radial homogeneity in high-performance liquid chromatography

被引:74
作者
Farkas, T
Sepaniak, MJ
Guiochon, G
机构
[1] UNIV TENNESSEE,DEPT CHEM,KNOXVILLE,TN 37996
[2] OAK RIDGE NATL LAB,DIV CHEM & ANALYT SCI,OAK RIDGE,TN 37831
关键词
flow velocity distribution; fluorescence detection; on-column; column efficiency;
D O I
10.1016/0021-9673(96)00107-0
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
The radial distribution of analyte molecules within an elution band in HPLC was determined by local, on-column, fluorescence detection at the column outlet. Several optical fiber assemblies were implanted in the exit frit at different points over the column cross-section and the fluorescence of a laser-dye analyte was measured. The individual elements of a diode array were used as independent detectors. The distribution of the mobile phase velocity across the column was measured for a number of standard size analytical HPLC columns of different efficiencies, operated at different mobile phase linear velocities. The dependence of the column efficiency on these profiles is discussed.
引用
收藏
页码:169 / 181
页数:13
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