Salt and Drought Stresses Induce the Aberrant Expression of microRNA Genes in Tobacco

被引：136
作者：
Frazier, Taylor P. [1 ]
Sun, Guiling [1 ]
Burklew, Caitlin E. [1 ]
Zhang, Baohong [1 ]
机构：
[1] E Carolina Univ, Dept Biol, Greenville, NC 27858 USA
来源：
MOLECULAR BIOTECHNOLOGY | 2011年 / 49卷 / 02期
关键词：
Abiotic stress; Drought; Salinity; microRNA; Gene regulation; Tobacco; SMALL RNAS; COMPUTATIONAL IDENTIFICATION; TRANSCRIPTION FACTOR; REGULATED MICRORNAS; PLANT MICRORNAS; HIGH-SALINITY; TARGET GENES; ARABIDOPSIS; MIRNAS; COLD;
D O I：
10.1007/s12033-011-9387-5
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Drought and salinity stresses significantly altered microRNA (miRNA) expression in a dose-dependent manner in tobacco. Salinity stress changed the miRNA expression levels from a 6.86-fold down-regulation to a 616.57-fold up-regulation. Alternatively, miRNAs were down-regulated by 2.68-fold and up-regulated 2810-fold under drought conditions. miR395 was most sensitive to both stresses and was up-regulated by 616 and 2810-folds by 1.00% PEG and 0.171 M NaCl, respectively. Salinity and drought stresses also changed the expression of protein-coding genes [alcohol dehydrogenase (ADH) and alcohol peroxidase (APX)]. The results suggest that miRNAs may play an important role in plant response to environmental abiotic stresses. Further investigation of miRNA-mediated gene regulation may elucidate the molecular mechanism of plant tolerance to abiotic stresses and has the potential to create a miRNA-based biotechnology for improving plant tolerance to drought and salinity stresses.
引用
收藏
页码：159 / 165
页数：7
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