Encapsulation of proteins by layer-by-layer adsorption of polyelectrolytes onto protein aggregates: Factors regulating the protein release

被引：115

作者：

Balabushevitch, NG

Sukhorukov, GB ^{[1
]}

Moroz, NA

Volodkin, DV

Larionova, NI

Donath, E

Mohwald, H

机构：

[1] Max Planck Inst Colloids & Interfaces, D-14476 Golm, Germany

[2] Moscow MV Lomonosov State Univ, Dept Chem, Moscow 119899, Russia

[3] Russian Acad Sci, Inst Crystallog, Moscow 117333, Russia

来源：

BIOTECHNOLOGY AND BIOENGINEERING | 2001年 / 76卷 / 03期

关键词：

protein encapsulation; polyelectrolytes; ultrathin organic films; protein aggregates; microcapsules; colloid; protein release;

D O I：

10.1002/bit.1184

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

A novel method of protein encapsulation is proposed. Preformed protein aggregates are covered with polyelectrolyte layers by means of layer-by-layer adsorption. The polyelectrolyte membrane prevents protein leakage out of the capsule. Using chymotrypsin as a model enzyme the capsule wall selective permeability was demonstrated for substrates and inhibitors of different molecular weight and solubility. (C) 2001 John Wiley & Sons, Inc.

引用

页码：207 / 213

页数：7

共 31 条

[1]

Berezin I.V., 1979, BIOKINETICS

[2] DETECTION OF LIGHT SIGNALS BY MICROENCAPSULATED CIS-CINNAMOYL CHYMOTRYPSIN [J].