Combining multiphoton and CARS microscopy for skin imaging

被引：0

作者：

Breunig, H. G. ^{[1
]}

Weinigel, M. ^{[1
]}

Lademann, J. ^{[2
]}

Sterry, W. ^{[2
]}

Latka, I. ^{[3
]}

Dietzek, B. ^{[3
]}

Popp, J.

Koenig, K. ^{[1
,4
]}

机构：

[1] JenLab GmbH, Campus A 1-2,66123 Saarbrucken & Schillerstr 1, D-07745 Jena, Germany

[2] Charite Hosp, Dept Dermatol Venerol Allergol, D-10117 Berlin, Germany

[3] Univ Jena, IPHT, D-07745 Jena, Germany

[4] Univ Saarland, Dept Biophoton & Laser Technol, D-66123 Saarbrucken, Germany

来源：

MULTIPHOTON MICROSCOPY IN THE BIOMEDICAL SCIENCES XI | 2011年 / 7903卷

关键词：

Multiphoton imaging; CARS microscopy; skin; STOKES-RAMAN SCATTERING; IN-VIVO; FLUORESCENCE; TOMOGRAPHY; BIOLOGY;

D O I：

10.1117/12.874969

中图分类号：

R318 [生物医学工程];

学科分类号：

0831 ;

摘要：

We present combined coherent anti Stokes Raman scattering and two-photon excited fluorescence microscopy, for label-free in vitro and in vivo imaging of skin with both chemical discrimination and subcellular resolution. In particular, we show how CARS microscopy is utilized to image lipid-rich structures inside the skin, preparing for a combined multiphoton and CARS imaging modality for biomedical research and skin imaging. As a further potential application the detection of topically applied oil on the skin is presented.

引用

页数：6

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