Establishment and characterization of a new canine mast cell tumor cell line

被引:27
作者
Ishiguro, T [1 ]
Kadosawa, T
Mori, K
Takagi, S
Okumura, M
Fujinaga, T
机构
[1] Hokkaido Univ, Grad Sch Vet Med, Dept Vet Clin Sci, Lab Vet Surg, Sapporo, Hokkaido 0600818, Japan
[2] Hokkaido Univ, Grad Sch Vet Med, Dept Biomed Sci, Lab Anat, Sapporo, Hokkaido 0600818, Japan
关键词
canine; cell line; mast cell tumor;
D O I
10.1292/jvms.63.1031
中图分类号
S85 [动物医学(兽医学)];
学科分类号
0906 ;
摘要
A new cell line (CoMS) was established from a 3-year-old male mongrel dog with mast cell tumor of the oral mucosa. coma. cells grow in suspension with a doubling time of 27.0 +/- 0.7 hr, The cytoplasmic granules were formalin-sensitive, showed diverse appearances in their ultrastructural findings and contained heparin proteoglycan and neutral protease chymase. Calcium ionophore A23187, substance P and concanavalin A caused significant histamine release from CoMS cells, while compound 48/80 failed to release histamine. This cell line will make an available source for studies on canine mast cell tumors.
引用
收藏
页码:1031 / 1034
页数:4
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