Thermo-irreversible glycol chitosan/hyaluronic acid blend hydrogel for injectable tissue engineering

被引:70
作者
Lee, Eun Joo [1 ,2 ]
Kang, Eunae [1 ]
Kang, Sun-Woong [2 ,3 ]
Huh, Kang Moo [1 ]
机构
[1] Chungnam Natl Univ, Dept Polymer Sci & Engn, 99 Daehak Ro, Daejeon 34134, South Korea
[2] Korea Inst Toxicol, Res Grp Biomimet Adv Technol, 141 Gajeong Ro, Daejeon 34114, South Korea
[3] Univ Sci & Technol, Human & Environm Toxicol Program, 217 Gajeong Ro, Daejeon 34113, South Korea
基金
新加坡国家研究基金会;
关键词
Glycol chitosan; Hyaluronic acid; Thermo-irreversible sol-gel; Cell binding affinity; Injectable tissue engineering; HYALURONIC-ACID; CHITOSAN; BIODEGRADATION; NANOPARTICLES; BIOMATERIALS; DELIVERY; GELATION;
D O I
10.1016/j.carbpol.2020.116432
中图分类号
O69 [应用化学];
学科分类号
081704 ;
摘要
Thermogels that undergo temperature-dependent sol-gel transition have recently attracted attention as a promising biomaterial for injectable tissue engineering. However, conventional thermogels usually suffer from poor physical properties and low cell binding affinity, limiting their practical applications. Here, a simple approach for developing a new thermogel with enhanced physical properties and cell binding affinity is proposed. This thermogel (AcHA/HGC) was obtained by simple blending of a new class of polysaccharide-based thermogel, N-hexanoyl glycol chitosan (HGC), with a polysaccharide possessing good cell binding affinity, acetylated hyaluronic acid (AcHA). Gelation of AcHA/HGC was initially triggered by the thermosensitive response of HGC and gradually intensified by additional physical crosslinking mechanisms between HGC and AcHA, resulting in thermo-irreversible gelation. Compared to the thermos-reversible HGC hydrogel, the thermo-irreversible AcHA/HGC hydrogel exhibited enhanced physical stability, mechanical properties, cell binding affinity, and tissue compatibility. These results suggest that our thermo-irreversible hydrogel is a promising biomaterial for injectable tissue engineering.
引用
收藏
页数:11
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