UV Raman spectroscopy -: A technique for biological and mineralogical in situ planetary studies

被引：72

作者：

Tarcea, Nicolae ^{[1
]}

Harz, Michaela ^{[1
]}

Roesch, Petra ^{[1
]}

Frosch, Torsten ^{[1
]}

Schmitt, Michael ^{[1
]}

Thiele, Hans ^{[2
]}

Hochleitner, R. ^{[4
]}

Popp, Juergen ^{[1
,3
]}

机构：

[1] Univ Jena, Inst Phys Chem, D-07743 Jena, Germany

[2] Kayser Threde GmbH, Munich, Germany

[3] Inst Photon Technolg eV, D-07745 Jena, Germany

[4] Mineralog Staatssammlung Munchen, Munich, Germany

来源：

SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY | 2007年 / 68卷 / 04期

关键词：

UV Raman spectroscopy; meteroites; microorganism;

D O I：

10.1016/j.saa.2007.06.051

中图分类号：

O433 [光谱学];

学科分类号：

0703 ; 070302 ;

摘要：

We report on the great advantages of using deep UV Raman system for in situ planetary applications. Among them are to be mentioned: (1) higher scattering efficiency compared to VIS-IR Raman excitation wavelengths, (11) electronic resonance effects which increase the intrinsically weak Raman signal thus improving the S/N ratio of the detected Raman signals and (111) spectral separation of Raman and fluorescence signals. All these advantages are making UV Raman a valuable technique for in situ planetary applications. Mineral as well as biological samples were analyzed using Raman deep UV excitation and the results are presented. For the mineral samples a comparison with excitation in the NIR-VIS spectral regions is made. The impact of fluorescence on Raman data acquisition at different laser excitation wavelengths is assessed. Making use of the resonance effects, spectra of microorganisms were recorded with a high S/N ratio, allowing afterwards a very precise identification and classification (to the strain level) of the measured samples. (c) 2007 Elsevier B.V. All rights reserved.

引用

页码：1029 / 1035

页数：7

共 46 条

[1] Gene and bacterial identification using high-throughput technologies: Genomics, proteomics, and phonemics [J].