Evaluation of nutrient agar for the culture of Mycobacterium tuberculosis using the microcolony detection method

被引：2
作者：
Satti, L. [1 ]
Abbasi, S. [2 ]
Faiz, U. [3 ]
机构：
[1] Combined Mil Hosp, Dept Pathol, Dera Ismail Khan, Pakistan
[2] Combined Mil Hosp, Dept Pathol, Kharian, Pakistan
[3] Fdn Univ Med Coll, Dept Microbiol, Rawalpindi, Pakistan
来源：
INTERNATIONAL JOURNAL OF TUBERCULOSIS AND LUNG DISEASE | 2012年 / 16卷 / 07期
关键词：
Mycobacterium tuberculosis; nutrient agar; culture; tuberculosis; DIAGNOSIS; COMPLEX; 7H11;
D O I：
10.5588/ijtld.11.0849
中图分类号：
R51 [传染病];
学科分类号：
100401 ;
摘要：
We evaluated nutrient agar using the microcolony detection method for the recovery of Mycobacterium tuberculosis on 37 acid-fast bacilli (AFB) positive sputum specimens, and compared it with conventional Lowenstein-Jensen (LJ) medium. Nutrient agar detected 35 isolates compared to 34 on LJ medium. The mean time to detection of mycobacteria on nutrient agar and 14 medium was respectively 9.6 and 21.4 days. The contamination rate on nutrient agar and LJ medium was respectively 5.4% and 2.7%. Nutrient agar detects M. tuberculosis more rapidly than LJ medium, and could be an economical, rapid culture method in resource-poor settings, provided our findings are confirmed by further studies.
引用
收藏
页码：908 / 910
页数：3
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