One-step PCR mediated strategy for the construction of conditionally expressed and epitope tagged yeast proteins

被引：84

作者：

Lafontaine, D ^{[1
]}

Tollervey, D ^{[1
]}

机构：

[1] EUROPEAN MOL BIOL LAB,D-69012 HEIDELBERG,GERMANY

来源：

NUCLEIC ACIDS RESEARCH | 1996年 / 24卷 / 17期

关键词：

D O I：

10.1093/nar/24.17.3469

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

With the availability of the complete yeast genomic sequence, techniques which allow the rapid functional analysis of genes of interest are of increasing importance. Here we report a technique which allows the initial characterisation of genes of interest, through the construction of conditionally expressed mutations for functional analyses and the generation of epitope-tagged fusion proteins for immuno-localisation and immuno-purification, entirely by PCR.

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页码：3469 / 3471

页数：3

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