Biodegradable Mg-6Zn Alloy Down-Regulation the NF-κB Signaling Pathway of Intestinal Epithelial Cells

Nuclear factor kappa B (NF-kappa B) signaling pathway plays an important role in the processes of immunity, inflammation, and cancer. As studies of magnesium (Mg)-zinc (Zn) alloy have increased, it becomes necessary to investigate effects of Mg-6Zn alloy on NF-kappa B signaling pathway. The Mg-6Zn alloy was investigated in both in vitro and in vivo experiments. The mRNA expression of P65, P50 and I kappa B alpha, and the protein expressions of pI kappa B alpha were tested using real time-PCR and western blot analysis. In the in vivo experiments, pins of Mg-6Zn alloy were implanted into the intestinal wall of SD rats. After 1 week, H and E staining and immunohistochemical analysis of NF-kappa B, was performed. Results of this study showed the expression of I kappa B alpha, pI kappa B alpha, P50 and P65 did not significantly fluctuate in the 20% and 40% concentrations of Mg-6Zn alloy extracts. In the 80% and 100% extracts, the mRNA expression of I kappa B alpha was higher than that in the 0% extract. The mRNA expression of P50 and P65 were significantly lower in the 60%, 80% and 100% extracts than those in the 0% extract. The protein level of pI kappa B alpha was lower in the 60%, 80% and 100% extracts. In the in vivo experiments, there were fewer inflammation and lower levels of expression of NF-kappa B in the Mg-6Zn alloy group than those in the control group. The NF-kappa B signal pathway of IEC-6 cells is sensitive to the concentration of the Mg-6Zn extracts. High concentrations extracts (60%, 80% and 100%) partly inhibit the NF-kappa B signal pathway. The I kappa B alpha is the main acting sit of Mg-6Zn extracts. High concentrations Mg-6Zn extracts increase levels of I kappa B alpha and decrease I kappa B alpha degradation.