In vivo expression technology identifies a type VI secretion system locus in Burkholderia pseudomallei that is induced upon invasion of macrophages

被引:197
作者
Shalom, Gil [1 ]
Shaw, Jonathan G. [1 ]
Thomas, Mark S. [1 ]
机构
[1] Univ Sheffield, Sch Med & Biomed Sci, Unit Infect & Immun, Sheffield S10 2RX, S Yorkshire, England
来源
MICROBIOLOGY-SGM | 2007年 / 153卷
关键词
D O I
10.1099/mic.0.2007/006585-0
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
The Gram-negative proteobacterium Burkholderia pseudomallei can survive and multiply within a variety of eukaryotic cells, including macrophages. This property is believed to be important for its ability to cause the disease melioidosis in a wide range of animal species, including humans. To identify determinants that are important for the ability of B. pseudomallei to survive within macrophages, in vivo expression technology (IVET) was employed. Several putative macrophage-inducible genes were identified that are likely to contribute to the virulence of B. pseudomallei, including three genes (tssH-5, tssl-5 and tssM-5) located within the same type VI secretion system cluster (tss-5), mntH, encoding a natural resistance-associated macrophage protein (NRAMP)-like manganese ion transporter, and a haem acquisition gene, bhuT The macrophage-inducibility of the tss-5 gene cluster was confirmed by reporter gene analysis. Construction of tssH-5 and bhuT null mutants indicated that expression of the tss-5 unit and the bhu operon were not required for intramacrophage survival. A further five tss units were identified within the B. pseudomallei genome that, together with tss-5, account for approximately 2.3 % of the total genome size. The presence of six type VI secretion systems in this organism is likely to be an important factor in making this bacterium such a versatile pathogen.
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收藏
页码:2689 / 2699
页数:11
相关论文
共 64 条
[1]   Genetic and transcriptional analysis of the siderophore malleobactin biosynthesis and transport genes in the human pathogen Burkholderia pseudomallei K96243 [J].
Alice, AF ;
López, CS ;
Lowe, CA ;
Ledesma, MA ;
Crosa, JH .
JOURNAL OF BACTERIOLOGY, 2006, 188 (04) :1551-1566
[2]   Bacterial iron homeostasis [J].
Andrews, SC ;
Robinson, AK ;
Rodríguez-Quiñones, F .
FEMS MICROBIOLOGY REVIEWS, 2003, 27 (2-3) :215-237
[3]   In vivo expression technology [J].
Angelichio, MJ ;
Carnilli, A .
INFECTION AND IMMUNITY, 2002, 70 (12) :6518-6523
[4]  
[Anonymous], 1989, Molecular Cloning
[5]   A second type III secretion system in Burkholderia pseudomallei:: who is the real culprit? [J].
Attree, O ;
Attree, I .
MICROBIOLOGY-SGM, 2001, 147 :3197-3199
[6]   Lessons from signature-tagged mutagenesis on the infectious mechanisms of pathogenic bacteria [J].
Autret, N ;
Charbit, A .
FEMS MICROBIOLOGY REVIEWS, 2005, 29 (04) :703-717
[7]   Actin-based motility of Burkholderia pseudomallei involves the Arp 2/3 complex, but not N-WASP and Ena/VASP proteins [J].
Breitbach, K ;
Rottner, K ;
Klocke, S ;
Rohde, M ;
Jenzora, A ;
Wehland, J ;
Steinmetz, I .
CELLULAR MICROBIOLOGY, 2003, 5 (06) :385-393
[8]  
CHAOWAGUL W, 1993, J INFECT DIS, V168, P1181, DOI 10.1093/infdis/168.5.1181
[9]   Biogenesis, architecture, and function of bacterial type IV secretion systems [J].
Christie, PJ ;
Atmakuri, K ;
Krishnamoorthy, V ;
Jakubowski, S ;
Cascales, E .
ANNUAL REVIEW OF MICROBIOLOGY, 2005, 59 :451-485
[10]   The type III secretion injectisome [J].
Cornelis, Guy R. .
NATURE REVIEWS MICROBIOLOGY, 2006, 4 (11) :811-825