Previous studies have demonstrated that carbon dioxide (CO2) laser treatment of dental enamel can inhibit subsequent sub-surface caries-like progression. Irradiation of dental enamel by specific wavelengths and fluences of CO2 laser light alters the chemical composition of the crystals, decomposing the carbonate component. The present study examined the effects of fluence and number of pulses on the surface dissolution kinetics of dental enamel following laser irradiation of the surface. The initial dissolution rate of dental enamel (5x5 mm blocks) in pH 4.5 acetate buffer was determined after laser irradiation by a pulsed CO2 laser at 9.3 mu m , with a pulse duration of 100 mu s, and 0-25 pulses per spot, with fluences of 0-8 J/cm(2). Maximum inhibition of dissolution occurred at fluences of 3-5 J/cm(2) coinciding with maximum carbonate loss, but the beneficial effect was negated at higher fluences as the surface chemistry changed. Irradiation by more than 5 pulses produced minimal further benefit.