Selection of reference genes for quantitative real-time PCR analysis of photosynthesis-related genes expression in Lilium regale

被引：11

作者：

Du, Wenkai ^{[1
,2
]}

Hu, Fengrong ^{[2
]}

Yuan, Suxia ^{[1
]}

Liu, Chun ^{[1
]}

机构：

[1] Chinese Acad Agr Sci, Inst Vegetables & Flowers, Beijing 100081, Peoples R China

[2] Nanjing Forestry Univ, Coll Landscape Architecture, Nanjing 210037, Jiangsu, Peoples R China

来源：

PHYSIOLOGY AND MOLECULAR BIOLOGY OF PLANTS | 2019年 / 25卷 / 06期

关键词：

Lilium regale; Reference gene; Quantitative real-time PCR; Photosynthesis; RT-PCR; NORMALIZATION; QUANTIFICATION; MODEL; VALIDATION; MUTANT; QPCR;

D O I：

10.1007/s12298-019-00707-y

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Photosynthesis is closely related to the growth of plants. A stable reference gene is fundamental for studies of the molecular mechanism of photosynthesis in Lilium regale. Therefore, it is very important to select a suitable reference gene for qRT-PCR analysis on genes of photosynthetic system, chlorophyll biosynthetic pathway and chloroplast development in Lilium regale. Three kinds of tissues, leaves and bulbs (abnormal leaves) of tissue culture plantlets and cotyledons of seedlings of the wild-type and mutant Lilium regale were selected as materials for qRT-PCR. Six housekeeping genes were selected as candidate genes from transcriptome sequencing data of the wild-type and yellow seedling lethal mutant of Lilium regale. Finally, the expression stability of six candidate reference genes was analyzed using geNorm, NormFinder, and BestKeeper software, the comparative increment Ct method, and the RefFinder program. The results showed that LrActin2 was the best reference gene for qRT-PCR analysis of photosynthesis-related genes expression in leaves of tissue culture plantlets and seedlings of Lilium regale. This study provided useful data for further research on molecular mechanism of photosynthesis in the Lilium.

引用

页码：1497 / 1506

页数：10

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