Meta-Analysis of Gene Expressions in Testicular Germ Cell Tumor Histologies

被引:10
作者
von Eyben, Finn Edler [1 ]
Parraga-Alava, Jorge [2 ,3 ]
机构
[1] Ctr Tobacco Control Res, DK-5230 Odense M, Denmark
[2] Univ Tecn Manabi, Fac Ciencias Informat, Portoviejo 130105, Ecuador
[3] Univ Santiago Chile, Dept Ingn Informat, Santiago 9170020, Chile
关键词
biomarkers; genes for pluripotency; meta-analysis; oncogenes; pathogenesis; RNA expression; testicular neoplasms; tumor suppressor genes; CARCINOMA IN-SITU; EPIGENETIC FEATURES; DOWN-REGULATION; STEM-CELLS; DIFFERENTIATION; TESTIS; METHYLATION; NEOPLASIA; PATHOGENESIS; TRANSITION;
D O I
10.3390/ijms21124487
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
There is no consensus as to how a precursor lesion, germ cell neoplasia in situ (GCNIS), develops into the histologic types of testicular germ cell tumor type II (TGCT). The present meta-analysis examined RNA expressions of 24 candidate genes in three datasets. They included 203 samples of normal testis (NT) and histologic types of TGCT. The Fisher's test for combinedpvalues was used for meta-analysis of the RNA expressions in the three datasets. The histologic types differed in RNA expression ofPRAME, KIT, SOX17, NANOG, KLF4, POU5F1, RB1, DNMT3B, andLIN28A(p< 0.01). The histologic types had concordant differences in RNA expression of the genes in the three datasets. Eight genes had overlap with a high RNA expression in at least two histologic types. In contrast, only seminoma (SE) had a high RNA expression ofKLF4and only embryonal carcinoma (EC) had a high RNA expression ofDNMT3B. In conclusion, the meta-analysis showed that the development of the histologic types of TGCT was driven by changes in RNA expression of candidate genes. According to the RNA expressions of the ten genes, TGCT develops from NT over GCNIS, SE, EC, to the differentiated types of TGCT.
引用
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页码:1 / 15
页数:15
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