Study of Interaction of Nanoparticles and Proteins in Taylor Dispersion Analysis

The diffusion coefficient ((4. 11 +/- 0. 78) x10(-11) m(2)/s) and hydrated radius ((6. 12 +/- 1. 21) nm) of FITC-DSA (fluorescein isothiocyanate conjugate dog albumin) were investigated and measured by combining Taylor dispersion analysis with laser induced fluorescence in microchip. The influence of size of gold nanoparticles (15, 30 and 50 nm) on the interactions between FITC-DSA and gold nanoparticles was studied. The preliminary result demonstrated that the binding of protein and gold nanoparticle displayed a size. dependent relationship. It was interesting that the 50 nm gold nanoparticles could enhance the FITC-DSA fluorescence in microchannel driven with pressure. This method was simple, fast, low sample consumption and high throughput and could be used to study the interaction of nanoparticles and proteins. Systematical study using this method would enable us to have a deep understanding of the toxicity of nanomaterials, and promote the development of safe nanomedicine.