Quantitative genotyping of single nucleotide polymorphism by single-molecule multi-color fluorescence resonance energy transfer

被引：3
作者：
Koh, Hye Ran
Han, Kyu Young
Jung, Jiwon
Kim, Seong Keun [1 ]
机构：
[1] Seoul Natl Univ, Dept Chem, Seoul 151747, South Korea
来源：
CHEMICAL COMMUNICATIONS | 2011年 / 47卷 / 37期
基金：
新加坡国家研究基金会;
关键词：
MITOCHONDRIAL-DNA HETEROPLASMY; DRUG RESPONSE; MUTATIONS; DISEASE; QUANTIFICATION; PROBES; LEVEL; SNPS; TOOL; LNA;
D O I：
10.1039/c1cc12737c
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
We developed a new single nucleotide polymorphism (SNP) genotyping method based on single-molecule multi-color fluorescence resonance energy transfer (FRET). We demonstrated that this new method uses less than 1 fmol of sample and is also highly quantitative with a detection level of 1% or lower in the minor allele fraction.
引用
收藏
页码：10362 / 10364
页数：3
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