Single-step direct measurement of amyloid fibrils stiffness by peak force quantitative nanomechanical atomic force microscopy

被引：210
作者：
Adamcik, Jozef [1 ]
Berquand, Alexandre [2 ]
Mezzenga, Raffaele [1 ]
机构：
[1] ETH, Inst Food Nutr & Hlth, CH-8092 Zurich, Switzerland
[2] Bruker Nano GmbH, D-68165 Mannheim, Germany
来源：
APPLIED PHYSICS LETTERS | 2011年 / 98卷 / 19期
关键词：
ASSEMBLED PEPTIDE NANOTUBES; PROTEINS;
D O I：
10.1063/1.3589369
中图分类号：
O59 [应用物理学];
学科分类号：
摘要：
We present an original application of a new atomic force microscopy mode called peak force tapping for the investigation of the mechanical properties of beta-lactoglobulin amyloid fibrils. The values of Young's modulus obtained by this technique are in perfect agreement with the indirect evaluation of fibrils stiffness obtained by combining polymer physics and topological statistical analysis on fibrils' structural conformations. This technique shows great promise in the estimation of the elastic properties of nanostructured objects relevant in biology, soft matter, and nanotechnology. (C) 2011 American Institute of Physics. [doi:10.1063/1.3589369]
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页数：3
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