Lactic acid bacteria secrete toll like receptor 2 stimulating and macrophage immunomodulating bioactive factors

被引：26

作者：

Ren, Chengcheng ^{[1
,2
,3
]}

Cheng, Lianghui ^{[1
,2
]}

Sun, Yue ^{[1
,2
]}

Zhang, Qiuxiang ^{[3
]}

de Haan, Bart J. ^{[1
,2
]}

Zhang, Hao ^{[3
]}

Faas, Marijke M. ^{[1
,2
]}

de Vos, Paul ^{[1
,2
]}

机构：

[1] Univ Groningen, Dept Pathol & Med Biol, Div Med Biol, Immunoendocrinol, Hanzepl 1, NL-9700 RB Groningen, Netherlands

[2] Univ Med Ctr Groningen, Hanzepl 1, NL-9700 RB Groningen, Netherlands

[3] Jiangnan Univ, Sch Food Sci & Technol, 1800 Lihu Rd, Wuxi 214122, Jiangsu, Peoples R China

来源：

JOURNAL OF FUNCTIONAL FOODS | 2020年 / 66卷

关键词：

Immunomodulation; Lactic acid bacteria; Secreted bioactive factor; Toll-like receptor; LACTOBACILLUS-RHAMNOSUS; SUPERNATANT; INFLAMMATION; MICE; RNA;

D O I：

10.1016/j.jff.2020.103783

中图分类号：

TS2 [食品工业];

学科分类号：

0832 ;

摘要：

Lactic acid bacteria (LAB) can support host health and crosstalks between ligands on bacterial cells and Toll-like receptors (TLR) are known as key inducers of these health effects. LAB-secreted bioactive factors also possess health-promoting properties but the underlying mechanisms remain to be identified. Here, we report that secreted factors of different LAB strains induce cytokine (IL-10 and TNF-alpha) production by THP1-differentiated human macrophages in a species- and strain-dependent manner. LAB-secreted products also drive immune-stimulating and anti-pathogenic M1-macrophage polarization. Moreover, bacterial supernatants induce species- and strain-dependent TLR2 activation. Several bacterial strains such as L. casei CCFM9 and L. brevis CCFM498 cannot activate TLR signaling, but their secreted products strongly activate TLR2. This indicates that LAB-secreted components may further boost the functional effects of bacterial strains. Our results confirm direct interactions of LAB-secreted components with TLRs, and provide novel insights in how LABs may provide immunemodulation.

引用

页数：10

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