Sequence analysis of cox1 and nad1 genes in Echinococcus granulosus G3 genotype in camels (Camelus dromedarius) from central Iran

被引：73
作者：
Sharbatkhori, Mitra [1 ,2 ,3 ]
Harandi, Majid Fasihi [4 ]
Mirhendi, Hossein [1 ,2 ]
Hajialilo, Elham [4 ]
Kia, Eshrat Beigom [1 ,2 ]
机构：
[1] Univ Tehran Med Sci, Sch Publ Hlth, Dept Med Parasitol & Mycol, Tehran, Iran
[2] Univ Tehran Med Sci, Natl Inst Hlth Res, Tehran, Iran
[3] Golestan Univ Med Sci, Sch Med, Dept Med Parasitol & Mycol, Gorgan, Iran
[4] Kerman Univ Med Sci, Dept Med Parasitol, Sch Med, Kerman, Iran
来源：
PARASITOLOGY RESEARCH | 2011年 / 108卷 / 03期
关键词：
MOLECULAR CHARACTERIZATION; CYSTIC ECHINOCOCCOSIS; TAXONOMIC REVISION; CERVID STRAIN; G1; SHEEP; EPIDEMIOLOGY; PHYLOGENY; LIVESTOCK; HUMANS;
D O I：
10.1007/s00436-010-2092-7
中图分类号：
R38 [医学寄生虫学]; Q [生物科学];
学科分类号：
07 ; 0710 ; 09 ; 100103 ;
摘要：
Nineteen hydatid cyst isolates collected from camels in central Iran were subjected to sequences analysis of mitochondrial cytochrome c oxidase subunit 1 (cox1) and NADH dehydrogenase subunit 1 (nad1) genes. A consensus sequence obtained containing 366 nucleotides for cox1 and 471 nucleotides for nad1 genes. Overall, the camel isolates indicated five different sequences in cox1 and nine in nad1 genes. The sequences analysis indicated that 26.3%, 42.1%, and 31.6% of isolates belonging to G1, G3, and G6 genotypes of Echinococcus granulosus, respectively. The isolates with G3 genotype indicated one cox1 sequence having 100% homology with reference G3 sequence (AN: M84663) and two different nad1 sequences, one having100% homology with reference G3 sequence (AN: AJ237634) and the other with a silent mutation (G to A) in position 279. The presence of G3 genotype (buffalo strain) of E. granulosus as dominant genotype in camels is emphasized. As G3 genotype has formerly been reported in human, the epidemiological role of camels is warranted in future surveys.
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收藏
页码：521 / 527
页数：7
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