Comparative Characterization of Porcine Mesenchymal Stem Cells Derived from Bone Marrow Extract and Skin Tissues

被引：1
作者：
Ock, Sun-A [2 ]
Jeon, Byeong-Gyun
Rho, Gyu-Jin [1 ]
机构：
[1] Gyeongsang Natl Univ, Coll Vet Med, Life Sci Res Inst, Jinju 660701, South Korea
[2] Gyeongsang Natl Univ, Inst Anim Med, Jinju 660701, South Korea
来源：
TISSUE ENGINEERING PART C-METHODS | 2010年 / 16卷 / 06期
关键词：
NUCLEAR TRANSFER; PROGENITOR CELLS; EXPRESSION; EMBRYOS; NANOG; SWINE; OCT-4; DIFFERENTIATION; PLURIPOTENCY; LINEAGES;
D O I：
10.1089/ten.tec.2010.0149
中图分类号：
Q813 [细胞工程];
学科分类号：
摘要：
Mesenchymal stem cells (MSCs) offer a great promise for regenerative medicine. Present study compared the characterization of porcine MSCs (pMSCs) derived from bone marrow extract with adult ear and fetal skin-derived cells on morphology, cell growth, alkaline phosphatase activity, proliferation ability, expression of cluster of differentiation (CD) markers (CD29, 45, and 90), cell cycle, protein and mRNA levels of Oct-4, Sox-2, and Nanog, and lineage differentiation ability. Skin-derived cells exhibited alkaline phosphatase activity and differentiation ability like pMSCs. pMSCs possessed a longer doubling time than skin-derived cells, and there was no difference in the ratio of G0/G1 phase between pMSCs and skin-derived cells. Except for CD29 and 90, all cells were found negative for CD45. Protein and mRNA expression of Oct-4, Sox-2 and Nanog were observed with similar intensity in all cells. Taken together, pMSCs and skin-derived cells revealed similar characteristics, and suggested the possible supportive role of skin-derived cells with MSCs for the regeneration of damaged tissues in cell-based therapies.
引用
收藏
页码：1481 / 1491
页数：11
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