Ultrasensitive Detection of SARS-CoV-2 Antibody by Graphene Field-Effect Transistors

被引：90
作者：
Kang, Hua [1 ,2 ]
Wang, Xuejun [1 ,2 ]
Guo, Mingquan [3 ]
Dai, Changhao [1 ,2 ]
Chen, Renzhong [1 ,2 ]
Yang, Lei [1 ,2 ]
Wu, Yanling [4 ]
Ying, Tianlei [4 ]
Zhu, Zhaoqin [3 ]
Wei, Dapeng [5 ]
Liu, Yunqi [2 ,6 ]
Wei, Dacheng [1 ,2 ,5 ]
机构：
[1] Fudan Univ, Dept Macromol Sci, State Key Lab Mol Engn Polymers, Shanghai 200433, Peoples R China
[2] Fudan Univ, Inst Mol Mat & Devices, Dept Mat Sci, Shanghai 200433, Peoples R China
[3] Fudan Univ, Shanghai Publ Hlth Clin Ctr, Dept Lab Med, Shanghai 201508, Peoples R China
[4] Fudan Univ, Sch Basic Med Sci, Key Lab Med Mol Virol, MOE,NHC,CAMS, Shanghai 200032, Peoples R China
[5] Univ Chinese Acad Sci, Chongqing Sch, Chongqing 400714, Peoples R China
[6] Chinese Acad Sci, Inst Chem, Beijing 100190, Peoples R China
来源：
NANO LETTERS | 2021年 / 21卷 / 19期
基金：
国家重点研发计划; 中国博士后科学基金; 中国国家自然科学基金;
关键词：
field-effect transistor; biosensor; SARS-CoV-2; spike Si protein; antibody; BIOSENSOR; DIAGNOSIS; VIRUS;
D O I：
10.1021/acs.nanolett.1c00837
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
The fast spread of SARS-CoV-2 has severely threatened the public health. Establishing a sensitive method for SARS-CoV-2 detection is of great significance to contain the worldwide pandemic. Here, we develop a graphene field-effect transistor (g-FET) biosensor and realize ultrasensitive SARS-CoV-2 antibody detection with a limit of detection (LoD) down to 10(-18) M (equivalent to 10(-6) g mL(-1)) level. The g-FETs are modified with spike S1 proteins, and the SARS-CoV-2 antibody biorecognition events occur in the vicinity of the graphene surface, yielding an LoD of similar to 150 antibodies in 100 mu L full serum, which is the lowest LoD value of antibody detection. The diagnoses time is down to 2 min for detecting clinical serum samples. As such, the g-FETs leverage rapid and precise SARS-CoV-2 screening and also hold great promise in prevention and control of other epidemic outbreaks in the future.
引用
收藏
页码：7897 / 7904
页数：8
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