Optimization of an enzyme-based multi-parameter biosensor for monitoring biogas processes

被引：3
作者：
Pilas, J. [1 ]
Mariano, K. [2 ]
Keusgen, M. [3 ]
Selmer, T. [1 ]
Schoening, M. J. [1 ,4 ]
机构：
[1] FH Aachen, Inst Nano & Biotechnol INB, Heinrich Mussmann Str 1, D-52428 Julich, Germany
[2] RMIT Univ, Sch Appl Sci, Bundoora, Vic 3083, Australia
[3] Univ Marburg, Inst Pharmaceut Chem, D-35037 Marburg, Germany
[4] Forschungszentrum Julich, Peter Grunberg Inst PGI 8, D-52425 Julich, Germany
来源：
EUROSENSORS 2015 | 2015年 / 120卷
关键词：
biogas; lactate; formate; bi-enzyme; diaphorase; multi-parameter sensor chip;
D O I：
10.1016/j.proeng.2015.08.702
中图分类号：
T [工业技术];
学科分类号：
08 ;
摘要：
An enzyme-based multi-parameter sensor chip for the simultaneous measurement of formate, D-and L-lactate is presented. Thereby, the combination of a diaphorase (DIA) from Clostridium kluyveri with different NAD+-dependent dehydrogenases enables the specific recognition of each compound. The amperometric detection is performed via monitoring of the oxidation current of enzymatically produced hexacyanoferrate(II) at +300 mV vs. Ag/AgCl. Chemical cross-linking with glutaraldehyde is used for enzyme immobilization. Thereby, the sensor signal is investigated at different concentrations of the cross-linker in the enzyme membrane. The obtained results indicate that by optimization of the immobilization matrix, the sensor performance can be improved. (C) 2015 The Authors. Published by Elsevier Ltd.
引用
收藏
页码：532 / 535
页数：4
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