3D-printed transmembrane glycoprotein cancer biomarker aptasensor

被引：36

作者：

Crevillen, Agustin G. ^{[1
,2
]}

Mayorga-Martinez, Carmen C. ^{[1
]}

Zelenka, Jaroslav ^{[3
]}

Rimpelova, Silvie ^{[3
]}

Ruml, Tomas ^{[3
]}

Pumera, Martin ^{[1
,4
,5
,6
,7
]}

机构：

[1] Univ Chem & Technol Prague, Ctr Adv Funct Nanorobots, Dept Inorgan Chem, Tech 5, Prague 16628 6, Czech Republic

[2] Spanish Distance Univ UNED, Fac Sci, Dept Analyt Sci, E-28040 Madrid, Spain

[3] Univ Chem & Technol, Dept Biochem & Microbiol, Tech 5, Prague 16628 6, Czech Republic

[4] Yonsei Univ, Dept Chem & Biomol Engn, 50 Yonsei Ro, Seoul 03722, South Korea

[5] China Med Univ, China Med Univ Hosp, Dept Med Res, 91 Hsueh Shih Rd, Taichung, Taiwan

[6] Brno Univ Technol, Cent European Inst Technol, Future Energy & Innovat Lab, Purkynova 656-123, Brno 61600, Czech Republic

[7] Mendel Univ Brno, Dept Food Technol, 3D Printing & Innovat Hub, Zemedelska 1, Brno 61300, Czech Republic

来源：

APPLIED MATERIALS TODAY | 2021年 / 24卷

关键词：

Additive manufacturing; Aptamer; Breast cancer cells; Biomarker; Sensor; MUC1; ACTIVATION;

D O I：

10.1016/j.apmt.2021.101153

中图分类号：

T [工业技术];

学科分类号：

08 ;

摘要：

A B S T R A C T Point-of-care, easy to manufacture, and low cost detection of cancer biomarkers is crucial for fighting the early stage disease. 3D printing allows delocalized printing at remote locations with updates/upgrades available globally via electronic files. Mucin 1 (MUC1) is a protein that is overexpressed in a number of epithelial cancers and is a key factor in advancement of the disease. Here we show the simple point of-care 3D printing fabrication of a MUC1 aptasensor using a nanocarbon/polymer filament. Efficacy of the 3D-printed aptasensor is demonstrated by monitoring the expression and release of MUC1 in breast cancer cell cultures. Such a simple, low cost, and easy to locally fabricate cancer biosensor will have a large impact on the field of cancer diagnostics. (c) 2021 Elsevier Ltd. All rights reserved.

引用

页数：5

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