Systems-wide temporal proteomic profiling in glucose-starved Bacillus subtilis

被引：144

作者：

Otto, Andreas ^{[1
]}

Bernhardt, Joerg ^{[1
]}

Meyer, Hanna ^{[2
]}

Schaffer, Marc ^{[1
]}

Herbst, Florian-A. ^{[1
]}

Siebourg, Juliane ^{[3
]}

Maeder, Ulrike ^{[1
,4
]}

Lalk, Michael ^{[2
]}

Hecker, Michael ^{[1
]}

Becher, Doerte ^{[1
]}

机构：

[1] Ernst Moritz Arndt Univ Greifswald, Inst Microbiol, D-17487 Greifswald, Germany

[2] Ernst Moritz Arndt Univ Greifswald, Inst Pharm, D-17487 Greifswald, Germany

[3] Swiss Fed Inst Technol, Dept Biosyst Sci & Engn, CH-4058 Basel, Switzerland

[4] Ernst Moritz Arndt Univ Greifswald, Interfac Inst Genet & Funct Genom, D-17487 Greifswald, Germany

来源：

NATURE COMMUNICATIONS | 2010年 / 1卷

关键词：

MEMBRANE PROTEOME; QUANTITATIVE-ANALYSIS; 2-COMPONENT SYSTEM; MESSENGER-RNA; PROTEINS; EXPRESSION; VISUALIZATION; PROTEOLYSIS; STARVATION; REGULON;

D O I：

10.1038/ncomms1137

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Functional genomics of the Gram-positive model organism Bacillus subtilis reveals valuable insights into basic concepts of cell physiology. In this study, we monitor temporal changes in the proteome, transcriptome and extracellular metabolome of B. subtilis caused by glucose starvation. For proteomic profiling, a combination of in vivo metabolic labelling and shotgun mass spectrometric analysis was carried out for five different proteomic subfractions (cytosolic, integral membrane, membrane, surface and extracellular proteome fraction), leading to the identification of similar to 52% of the predicted proteome of B. subtilis. Quantitative proteomic and corresponding transcriptomic data were analysed with Voronoi treemaps linking functional classification and relative expression changes of gene products according to their fate in the stationary phase. The obtained data comprise the first comprehensive profiling of changes in the membrane subfraction and allow in-depth analysis of major physiological processes, including monitoring of protein degradation.

引用

页数：9

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