Synergism between wild-type Bacillus thuringiensis subsp. israelensis and B. sphaericus strains: A study based on isobolographic analysis and histopathology

被引:10
作者
Sreshty, M. Asha Latha [1 ]
Kumar, K. Pranay [1 ]
Murty, U. S. N. [1 ]
机构
[1] Indian Inst Chem Technol, Div Biol, Hyderabad 500607, Andhra Pradesh, India
关键词
Mosquito control; Bacillus spores; Synergism; Resistance; Isobolographic analysis; Histopathology; CULEX-QUINQUEFASCIATUS DIPTERA; HIGH-LEVEL; INSECTICIDE RESISTANCE; FIELD POPULATIONS; AEDES-AEGYPTI; IN-FIELD; TOXINS; GENES; RECOMBINANT; SELECTION;
D O I
10.1016/j.actatropica.2010.12.012
中图分类号
R38 [医学寄生虫学]; Q [生物科学];
学科分类号
07 ; 0710 ; 09 ; 100103 ;
摘要
Prevention is the best resistance management strategy in integrated vector control programs. Combined use of insecticides of different classes that interact synergistically and show multi-site actions within the insect is recognized as a potential key strategy to be implemented even before the onset of resistance. The present study is aimed at harvesting the benefits of synergism between the wild-type Bacillus thuringiensis subsp. israelensis-H14 (Bti) and Bacillus sphaericus-2362 (Bs) strains by evaluating six different combinations of mixtures toxic to Aedes and Culex mosquito larvae. Isobolographic analysis was performed to distinguish the synergistic combinations of Bti and Bs, followed by determination of the degree of synergism through synergy and improvement factors. Furthermore, the speed of activity of Bs when combined with Bti is studied by histopathological investigations on the fate of midgut muscles of mosquito larvae upon exposure to individual wild-type strains as well as their mixtures. (C) 2011 Elsevier B.V. All rights reserved.
引用
收藏
页码:14 / 20
页数:7
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